Cloning, characterization and computational analysis of the 5′ regulatory region of ovine glucose 6-phosphate dehydrogenase gene
Cloning, characterization and computational analysis of the 5′ regulatory region of ovine glucose 6-phosphate dehydrogenase gene
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DOI：
10.1016/j.cbpb.2007.04.001
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Rogdakis, Emmanuel
中科院分区：
文献类型：
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作者：
Laliotis, George P.;Bizelis, Iosif;Rogdakis, Emmanuel
为了更好地了解绵羊葡萄糖-6-磷酸脱氢酶（G6PD）基因启动子区的结构和功能，采用基因组步移法分离并测序了一个1628 bp的片段，该片段包含G6PD基因的5 '调控区。在sifico分析的序列显示了许多保守的区块和功能与其他已知的哺乳动物G6PD启动子区。分析还揭示了存在一个TATA盒、三个GC盒、两个E盒以及刺激蛋白1（Sp 1）和激活蛋白2（AP 2）的几个结合位点。此外，参与调节脂肪生成的元素，如USF，（上游刺激因子）（热冲击系数），F2F（催乳素受体），RAR视黄酸受体（应力响应元件），RORa（维甲酸相关孤儿受体α），加塔（加塔结合因子），RFX（调节因子X），SREBP（固醇调节元件结合蛋白）检测金属元素蛋白（Metal Element Protein）、CREB（Insulin Receptor）、PRE（Progesterone Receptor）和HNF 4（Hepatic Nuclear Factor 4）。最重要的调控基序被认为是保守的，相比，在人类和小鼠的同行。然而，也注意到一些差异，可能表明反刍动物和非反刍动物物种之间的G6PD基因的转录调控的差异。(c)2007年爱思唯尔公司All rights reserved.
To better understand the structure and the function of ovine glucose 6-phosphate dehydrogenase (G6PD) promoter region, a genome-walking procedure was followed to isolate and sequence a 1628 bp fragment, containing the 5' regulatory region of the G6PD gene. In sifico analysis of the sequence showed many conserved blocks and features with other known mammalian G6PD promoter regions. The analysis also revealed the presence of one TATA box, three GC boxes, two E-boxes and several binding sites for Stimulating Protein 1 (Sp 1) and Activator Protein 2 (AP2). Moreover, elements involved in the regulation of lipogenesis like USF (Upstream stimulating factor), HSF (Heat Shock Factor), F2F (Prolactin receptor), RAR (Retinoid Acid Receptor), STRE (STress Response Element), RORa (Retinoid related Orphan Receptor alpha), GATA (GATA binding factor), RFX (Regulatory Factor X), SREBP (Sterol Regulatory Element Binding Protein), MEP (Metal Element Protein), CREB (insulin receptor), PRE (Progesterone receptor), and HNF4 (Hepatic Nuclear Factor 4) were detected. The most important regulatory motifs were found to be conserved as compared to those in human and mouse counterparts. However, some differences were noted, likely indicating differences in the transcription regulation of G6PD gene between ruminant and non-ruminant species. (c) 2007 Elsevier Inc. All rights reserved.