Comparative study of the pln locus of the quorum-sensing regulated bacteriocin-producing L-plantarum J51 strain

Comparative study of the pln locus of the quorum-sensing regulated bacteriocin-producing L-plantarum J51 strain
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DOI:
10.1016/j.ijfoodmicro.2008.08.004
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发表时间:
2008-12-10
影响因子:
5.4
通讯作者:
Torres, Carmen
Torres, Carmen
中科院分区:
农林科学1区
文献类型:
--
作者:
Navarro, Laura;Rojo-Bezares, Beatriz;Torres, Carmen

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植物乳杆菌(LactobacillusplantarumJ51)是从一种葡萄酒中分离得到的一株具有抗菌活性的菌株。这些特性赋予了L. plantarum J51在葡萄酒微生物学和细菌素生产的研究中具有很高的兴趣。本工作观察到细菌素的产生受“群体感应”机制的调控,并对细菌素产生菌L. plantarum J51是完全特征化的。对20,667bp的片段进行了完全测序(GenBank登录号DQ 340868),并显示了五个操纵子(plNC 8 β α c、plnLR样、plnABCD. plnanosis,plnGHSTUVW)和一个含有一个假定操纵子的新区域,该操纵子具有三个新的orfs,可以编码一个假定的两肽细菌素。(c)2008 Elsevier B.V.保留所有权利。
Lactobacillus plantarum J51 strain was isolated from a Rioja red wine and it showed bacteriocin activity against a wide range of lactic acid bacteria of oenological importance. These characteristics conferred L. plantarum J51 a high interest both in wine microbiology and in the study of bacteriocin production. In this work the bacteriocin production regulated under the "quorum-sensing" mechanism is observed and the pin locus of the bacteriocin-producing L. plantarum J51 is fully characterized. A 20,667 bp fragment was completely sequenced (GenBank accession number DQ340868), and showed five operons (plNC8 beta alpha c, plnLR-like, plnABCD. plnEFI, plnGHSTUVW) and a new region containing a putative operon with three new orfs that could encode a putative two-peptide bacteriocin. (c) 2008 Elsevier B.V. All rights reserved.