NO metabolites accumulate in erythrocytes in proportion to carbon dioxide and bicarbonate concentration.
NO metabolites accumulate in erythrocytes in proportion to carbon dioxide and bicarbonate concentration.
复制标题
NO 代谢物在红细胞中的积累与二氧化碳和碳酸氢盐浓度成比例。
DOI：
10.1152/ajpheart.2000.279.2.h852
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Hintze,TH
中科院分区：
文献类型：
--
作者：
Recchia,FA;Vogel,TR;Hintze,TH
目前尚不清楚血浆（pNOx）和红细胞（eNOx）中NO代谢物（NOx）浓度之间的比率是否恒定或与血液的化学参数相关。我们测量了7只狗的主动脉、冠状窦和腿静脉的19个血液样本的pH、Po 2和Pco 2，并计算了碳酸氢盐浓度。然后通过离心分离红细胞并用蒸馏水裂解，并将裂解物以50 kDa的分子截留值超滤以除去血红蛋白。在血浆和超滤液中测量NOx。红细胞中的NOx浓度较高，eNOx/pNOx范围为4.38至14.60。eNOx/pNOx的自然对数与Pco 2（r= 0.70，P < 0.001）和HCO 3浓度（r= 0.72，P < 0.001）呈直线相关。这些结果表明，第一次，血浆NOx不能被认为是血液中的总NOx的一个恒定的分数，但变化显着的CO2/碳酸氢盐浓度成比例。为了防止低估跨器官的NOx的静脉-动脉差异，应在全血中测量NOx。
It is not known whether the ratio between the concentrations of NO metabolites (NOx) in plasma (pNOx) and in erythrocytes (eNOx) is constant or correlates with chemical parameters of the blood. We measured pH, Po2, and Pco2and calculated bicarbonate concentration in 19 blood samples from the aorta, coronary sinus, and leg veins of 7 dogs. Erythrocytes were then separated by centrifugation and lysed with distilled water, and the lysate was ultrafiltered with a molecular cutoff of 50 kDa to remove the hemoglobin. NOx were measured in plasma and in the ultrafiltrate. NOx concentration was higher in erythrocytes, with eNOx/pNOx ranging from 4.38 to 14.60. Linear and significant correlations were found between the natural logarithm of eNOx/pNOx and Pco2(r= 0.70,P< 0.001) or bicarbonate concentration (r= 0.72,P< 0.001). These results demonstrate, for the first time, that plasma NOx cannot be considered as a constant fraction of the total NOx in blood but varies dramatically in proportion to the CO2/bicarbonate concentration. To prevent an underestimation of venous-arterial difference of NOx across organs, NOx should be measured in whole blood.