A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control
A multiscale analysis of early flower development in Arabidopsis provides an integrated view of molecular regulation and growth control
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DOI：
10.1016/j.devcel.2021.01.019
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发表时间：
2021-02-22
影响因子：
11.8
通讯作者：
Traas, Jan
中科院分区：
文献类型：
--
作者：
Refahi, Yassin;Zardilis, Argyris;Traas, Jan
我们分析了拟南芥花发育早期基因调控与生长之间的联系。从延时图像开始，我们生成了早期花发育的4D图谱，包括细胞谱系、细胞生长速率和调控基因的表达模式。这些信息是在基于web的平台MorphoNet中引入的。使用计算模型，我们发现基于文献的分子网络只能解释少数基因表达模式。通过增加对单个基因的调节假设，这一点得到了极大的改善。将生长与多种调节因子的组合表达联系起来，导致了一组关于个体基因在形态发生中的作用的假设。这证实了中心因子LEAFY是异质生长的潜在调节因子，这一结论得到了叶片突变体生长模式量化的支持。通过提供一个综合的观点，这个地图集应该代表了向花卉发育的机制模型迈出的基本一步。
We have analyzed the link between the gene regulation and growth during the early stages of flower development in Arabidopsis. Starting from time-lapse images, we generated a 4D atlas of early flower development, including cell lineage, cellular growth rates, and the expression patterns of regulatory genes. This information was introduced in MorphoNet, a web-based platform. Using computational models, we found that the literature-based molecular network only explained a minority of the gene expression patterns. This was substantially improved by adding regulatory hypotheses for individual genes. Correlating growth with the combinatorial expression of multiple regulators led to a set of hypotheses for the action of individual genes in morphogenesis. This identified the central factor LEAFY as a potential regulator of heterogeneous growth, which was supported by quantifying growth patterns in a leafy mutant. By providing an integrated view, this atlas should represent a fundamental step toward mechanistic models of flower development.