Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum.
Gestation-specific reference intervals for comprehensive spot urinary steroid hormone metabolite analysis in normal singleton pregnancy and 6 weeks postpartum.
复制标题
DOI：
10.1186/s12958-015-0100-6
复制
发表时间：
2015-09-04
期刊：
影响因子：
--
通讯作者：
Gennari-Moser C
中科院分区：
文献类型：
--
作者：
Mistry HD;Eisele N;Escher G;Dick B;Surbek D;Delles C;Currie G;Schlembach D;Mohaupt MG;Gennari-Moser C
正常妊娠取决于类固醇激素浓度的明显适应。虽然近年来，这些激素在怀孕的理解有所改善，解释是阻碍了不足的参考值。我们的目的是建立正常单胎妊娠和产后6周尿类固醇激素水平的妊娠特异性参考区间。横断面多中心观察性研究。2008年至2013年，在瑞士（伯尔尼）、苏格兰（格拉斯哥）和奥地利（格拉兹）的3所大学医院招募了女性。在妊娠第11、20和28周以及产后约6周时，从参加常规产前检查诊所的正常妊娠的健康女性（年龄，16-45岁;平均年龄，31岁）中采集点尿。使用气相色谱质谱法分析尿类固醇激素水平。还通过常规分析测量肌酐并用于标准化。根据结果，计算每个妊娠期和产后6周每种激素代谢物的参考区间。还观察到几种类固醇激素在妊娠期和产后之间的这些浓度变化，并随后对指标类固醇激素提出了变化。现在，整个妊娠期和产后早期尿类固醇激素的正常妊娠特异性参考值可用于促进妊娠期和产后早期类固醇激素代谢的临床管理和研究方法。
Normal pregnancy depends on pronounced adaptations in steroid hormone concentrations. Although in recent years, the understanding of these hormones in pregnancy has improved, the interpretation is hampered by insufficient reference values. Our aim was to establish gestation-specific reference intervals for spot urinary steroid hormone levels in normal singleton pregnancies and 6 weeks postpartum. Cross-sectional multicentre observational study. Women recruited between 2008 and 2013 at 3 University Hospitals in Switzerland (Bern), Scotland (Glasgow) and Austria (Graz). Spot urine was collected from healthy women undergoing a normal pregnancy (age, 16–45 years; mean, 31 years) attending routine antenatal clinics at gestation weeks 11, 20, and 28 and approximately 6 weeks postpartum. Urine steroid hormone levels were analysed using gas-chromatography mass spectrometry. Creatinine was also measured by routine analysis and used for normalisation. From the results, a reference interval was calculated for each hormone metabolite at each trimester and 6 weeks postpartum. Changes in these concentrations between trimesters and postpartum were also observed for several steroid hormones and followed changes proposed for index steroid hormones. Normal gestation-specific reference values for spot urinary steroid hormones throughout pregnancy and early postpartum are now available to facilitate clinical management and research approaches to steroid hormone metabolism in pregnancy and the early postpartum period.