DEVELOPMENTALLY-REGULATED EXPRESSION OF A NOVEL 59-KDA PRODUCT OF THE MAJOR SURFACE PROTEASE (MSP OR GP63) GENE FAMILY OF LEISHMANIA-CHAGASI
DEVELOPMENTALLY-REGULATED EXPRESSION OF A NOVEL 59-KDA PRODUCT OF THE MAJOR SURFACE PROTEASE (MSP OR GP63) GENE FAMILY OF LEISHMANIA-CHAGASI
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DOI：
10.1074/jbc.270.15.8884
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发表时间：
1995-04-14
影响因子：
4.8
通讯作者：
DONELSON, JE
中科院分区：
文献类型：
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作者：
ROBERTS, SC;WILSON, ME;DONELSON, JE
所有种类的利什曼原虫都表达一种主要的表面蛋白酶（Msp或gp 63），该蛋白酶在其生命周期的几个步骤中促进寄生虫与其环境的相互作用。查氏利什曼原虫中的msp基因家族包含三类基因，其mRNA在寄生虫生长期间差异表达，对数期（低感染性）前鞭毛体仅表达63-kDa版本的Msp，而稳定期（高感染性）前鞭毛体表达63-和59-kDa Msp。59-和63-kDa蛋白质在丙烯酰胺凝胶上的不同迁移不是由于N-连接糖基化或膜锚的差异。利什曼原虫的质粒转染表明，mspS 2的固定基因类编码59 kDa的蛋白质。当寄生虫变弱时，在体外培养约12周后，稳定期前鞭毛体中59-kDa蛋白的表达停止。减毒的寄生虫可以通过小鼠传代，然后在体外传代几次回收的前鞭毛体来刺激重新表达59-kDa Msp。无鞭毛体表达Msp蛋白的另一个子集。因此，mspS 2的59-kDa产物仅在稳定期前鞭毛体中表达，并且仅在最近暴露于哺乳动物宿主细胞中遇到的环境变化后表达。
All species of Leishmania express a major surface protease (Msp or gp63) that facilitates the interactions of the parasite with its environment at several steps in its life cycle. The msp gene family in Leishmania chagasi contains three classes of genes whose mRNAs are differentially expressed during parasite growth, Logarithmic phase (low infectivity) promastigotes express only 63-kDa versions of Msp, whereas stationary phase (high infectivity) promastigotes express both 63- and 59-kDa Msps. The different migrations of the 59- and 63-kDa proteins on acrylamide gels are not due to differences in N-linked glycosylation or the membrane anchor. Plasmid transfections of Leishmania demonstrate that mspS2 of the stationary gene class encodes a 59 kDa protein. Expression of the 59-kDa protein in stationary phase promastigotes ceases after about 12 weeks of in vitro cultivation when the parasites become attenuated. Attenuated parasites can be stimulated to re express the 59-kDa Msp by passage through mice followed by several in vitro passages of recovered promastigotes. Amastigotes express yet another subset of Msp proteins. Thus, the 59-kDa product of mspS2 is expressed only in stationary phase promastigotes and only after recent exposure to environmental changes encountered in the mammalian host cell.