Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation
Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation
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DOI：
10.1007/s00253-012-3876-8
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发表时间：
2012-05-01
影响因子：
5
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Nakanishi, Akihito;Bae, Jun Gu;Ueda, Mitsuyoshi
从白腐菌Trametes sp.中分离和克隆了一个漆酶I基因。Ha1.漆酶I含有10个内含子和一个原始的分泌信号序列。通过重叠聚合酶链式反应制备不含内含子的漆酶I，将其插入酵母表达载体pULD1，用于酵母细胞表面展示。用2，2‘-azino-bis(3-ethylbenzthiazoline-6-sulphonic酸(ABTS)对一株展示漆酶I的酵母作为全细胞生物催化剂的氧化活性进行了检测，结果表明构建的酵母具有较高的氧化活性。用ABTS显示漆酶I的酵母对水热处理稻草进行前处理后，将内切葡聚糖酶、纤维二糖水解酶和β-葡萄糖苷酶共表达的酵母与HPR进行发酵。经显示漆酶I的酵母处理的HPR发酵活性是对照酵母的1.21倍。结果表明，显示漆酶I的酵母与ABTS共表达内切葡聚糖酶、纤维二糖水解酶和β-葡萄糖苷酶直接发酵纤维素酶是有效的。
A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and beta-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and beta-glucosidase.