Effects of Gegen Qinlian decoction on microbial growth in simulated gastric and intestinal liquid.
Effects of Gegen Qinlian decoction on microbial growth in simulated gastric and intestinal liquid.
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DOI：
10.13381/j.cnki.cjm.202002001
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发表时间：
2020-01-01
期刊：
影响因子：
--
通讯作者：
Hui, H. Y.
中科院分区：
文献类型：
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作者：
He, Yun-shan;Tang, Yuan;Hui, H. Y.
目的：探讨葛根芹连汤对模拟胃肠液中微生物生长的影响。方法：在模拟肠液中加入葛根芹连汤1g/m L，在37℃下加入适量的金黄色葡萄球菌、大肠杆菌、沙门氏菌、需氧菌、黑曲霉、米曲霉、青霉菌、汉森变形杆菌消化，分别于0、1、2、4h取消化液1m L进行平板计数。结果：葛根七连汤对金黄色葡萄球菌、大肠埃希菌、沙门氏菌、产气杆菌、黑曲霉、米曲霉、青霉菌均有较强的抑制作用，而对汉森变形杆菌的作用则是先促进后抑制。当反应时间为2小时时，对4种真菌的抑制作用最明显。葛根芹连汤对模拟胃液中的金黄色葡萄球菌、大肠埃希菌、沙门氏菌、黑曲霉、青霉菌、汉森变形杆菌有一定的抑制作用，对铜绿假单胞菌无明显抑制作用。结论：葛根芹连汤对模拟胃肠液中的金黄色葡萄球菌、大肠埃希菌、沙门氏菌、黑曲霉、青霉菌、汉森变形杆菌有一定的抑制作用。在模拟肠液中对产气气杆菌有明显的抑制作用，在模拟胃液中无明显抑制作用。
Objective: To explore the effect of Gegen Qinlian decoction on microbial growth in simulated gastric and intestinal liquid. Methods: Gegen Qinlian decoction at a concentration of 1 g/mL was added into the simulated enteric juice, and the digestive juice were added appropriate amount of Staphylococcus aureus, Escherichia coli, Salmonella, Aerobacter, Aspergillus niger, Aspergillus oryzae, Penicillium, and Debaryomyces hansenii for digestion at 37°C. At 0 h, 1 h, 2 h and 4 h respectively, 1 mL of the digestive juice was drawn for dilution plate counting. Results: Gegen Qinilian decoction had a strong inhibitory effect on Staphylococcus aureus, Escherichia coli, Salmonella, Aerobacter aerogenes, Aspergillus niger, Aspergillus oryzae and Penicillium, while the impact on Debaryomyces hansenii was first promotive then inhibitory. The inhibitory effect on the four fungi were the most significant when the reaction time was 2 hours. Gegen Qinlian decoction had certain inhibitory effect on Staphylococcus aureus, Escherichia coli, Salmonella, Aspergillus niger, Penicillium, and Debaryomyces hansenii in simulated gastric juice, but had no obvious inhibitory effect on Aerobacter aeruginosa. Conclusion: Gegen Qinlien decoction has certain inhibitory effect on Staphylococcus aureus, Escherichia coli, Salmonella, Aspergillus niger, Penicillium, and Debaryomyces hansenii in simulated gastric and intestinal fluids. It has obvious inhibitory effect on Aerobacter aerogenes in the simulated intestinal fluid, but no obvious inhibitory effect in the simulated gastric juice.