Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes.
Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes.
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通过与两个必需基因互补的外部引导序列抑制大肠杆菌的活力。
DOI：
10.1073/pnas.121180398
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发表时间：
2001
影响因子：
11.1
通讯作者：
Altman,S
中科院分区：
文献类型：
--
作者：
McKinney,J;Guerrier-Takada,C;Wesolowski,D;Altman,S
窄谱抗菌活性旨在减少两种必需基因的表达，一种编码 RNase P 蛋白亚基（C5 蛋白），另一种编码促旋酶（促旋酶 A）。在这两种情况下，外部引导序列 (EGS) 都被设计为与任一 mRNA 复合。使用 EGS 技术，微生物活力水平降低至野生型菌株的 10% 以下。当一起使用时，EGS 具有累加性，并且取决于攻击促旋酶 A mRNA 时配对的核苷酸数量。就旋转酶 A 而言，15 聚体 EGS 中的三个未配对的核苷酸仍然有利于 EGS 的完全抑制，但五个未配对的核苷酸则不然。
Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (EGS) have been designed to complex with either mRNA. Using the EGS technology, the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA. In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.