Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains
Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains
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DOI：
10.1128/jcm.43.2.703-710.2005
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发表时间：
2005-02-01
影响因子：
9.4
通讯作者：
Wang, L
中科院分区：
文献类型：
--
作者：
Beutin, L;Tao, J;Wang, L
对33株O15群大肠杆菌的O：H血清型和毒力标志物进行了研究，并检测了O抗原特异性基因wzx和wzy。这些菌株来自9个不同的国家，来自健康或患病的人和动物以及食品，并在1941年至2003年期间分离出来。根据毒力标志物和临床资料，可以将菌株分为不同的病原群，如泌尿系致病性大肠杆菌、肠致病性大肠埃希菌、产志贺毒素的大肠杆菌和肠聚集性大肠杆菌。H血清学分型和鞭毛蛋白(Flic)基因分型结果表明，鞭毛蛋白(Flc)基因具有不同的H型，某些H型与毒力标记和基因分型密切相关。发现了病原群。O-抗原基因簇的核苷酸序列分析揭示了O15抗原生物合成的可能基因。为了敏感和特异地检测O15抗原特异性基因wzx和wzy，开发了聚合酶链式反应方法。在33个O15菌株中发现了高度的致病类型多样性，这与O15抗原特异性基因的高度相似性形成了鲜明对比。这可能表明O15决定簇已经通过水平基因转移传播到了一些遗传上无关的大肠杆菌菌株。
A collection of 33 Escherichia coli serogroup O15 strains was studied with regard to O:H serotypes and virulence markers and for detection of the O-antigen-specific genes wzx and wzy. The strains were from nine different countries, originated from healthy or diseased humans and animals and from food, and were isolated between 1941 and 2003. On the basis of virulence markers and clinical data the strains could be split into different pathogroups, such as uropathogenic E. coli, enteropathogenic E. coli, Shiga toxin-producing E. coli, and enteroaggregative E. coli. H serotyping and genotyping of the flagellin (fliC) gene revealed It different H types and a close association between certain H types, virulence markers, and. pathogroups was found. Nucleotide sequence analysis of the O-antigen gene cluster revealed putative genes for biosynthesis of O15 antigen. PCR assays were developed for sensitive and specific detection of the O15-antigen-specific genes wzx and wzy. The high pathotype diversity found in the collection of 33 O15 strains contrasted with the high level of similarity found in the genes specific to the O15 antigen. This might indicate that the O15 determinant has been spread by horizontal gene transfer to a number of genetically unrelated strains of E. coli.