Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific.
Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific.
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低密度脂蛋白摄取显示了狗的肝细胞表型，但不具有特异性。
DOI：
10.1089/scd.2015.0054
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发表时间：
2016
影响因子：
4
通讯作者：
Gow AG
中科院分区：
文献类型：
--
作者：
Gow AG
低密度脂蛋白（LDL）摄取是用于证明干细胞分化后肝细胞样功能的众多测试之一。已经描述了两种化合物LDL和乙酰化LDL（AcLDL）的使用，尽管每种化合物具有不同的摄取机制。从犬中收获三种原代肝细胞培养物和三组间充质基质细胞（MSC）培养物，来源于脂肪组织和骨髓。将这些细胞与市售的人和小鼠骨髓来源的MSC进行比较。通过基因表达和免疫荧光在所有原代肝细胞培养物、未分化的犬骨髓间充质干细胞和犬脂肪间充质干细胞中证实LDL受体表达。未分化的人和小鼠骨髓MSC也表达LDL受体。在体外，犬肝细胞采取了标记的LDL，但不是AcLDL。所有未分化的MSC都能摄取LDL，但不摄取AcLDL。总之，LDL而不是AcLDL是犬肝细胞样表型的检测，但这不是组织或种属特异性的，因此，在检测MSC向肝细胞分化的证据时，不是提供信息的检测。
Low-density lipoprotein (LDL) uptake is one of a number of tests used to demonstrate hepatocyte-like function after stem cell differentiation. Use of two compounds, LDL and acetylated LDL (AcLDL), has been described despite each having different mechanisms of uptake. Three primary hepatocyte cultures and three sets of mesenchymal stromal cell (MSC) cultures, derived from both adipose tissue and bone marrow, were harvested from dogs. Those cells were compared to commercially available human and mouse bone marrow-derived MSCs. LDL receptor expression was demonstrated by gene expression and immunofluorescence in all primary hepatocyte cultures, undifferentiated canine bone marrow MSCs and canine adipose MSCs. Undifferentiated human and mouse bone marrow MSCs also expressed the LDL receptor. In vitro, canine hepatocytes took up labeled LDL, but not AcLDL. All undifferentiated MSCs took up LDL, but not AcLDL. In conclusion, LDL and not AcLDL is a test of canine hepatocyte-like phenotype, but this is not tissue or species specific and, therefore, is not informative assay when testing proof of MSC to hepatocyte differentiation.
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