Probiotic supplementation reduces the risk of bacterial translocation in experimental short bowel syndrome
Probiotic supplementation reduces the risk of bacterial translocation in experimental short bowel syndrome
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DOI：
10.1053/jpsu.2002.32256
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发表时间：
2002-05-01
影响因子：
2.4
通讯作者：
Aldazabal, P
中科院分区：
文献类型：
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作者：
Eizaguirre, I;Urkia, NG;Aldazabal, P
背景/目的：益生菌是通过胃肠道存活的活生物体，对宿主具有有益作用。乳酸杆菌和双歧杆菌已被推荐用于降低胆固醇，急性腹泻，预防癌症或炎症性肠病。另一方面，大量肠切除术后，细菌过度生长是频繁的，有利于细菌移位（BT）。尚未研究乳酸双歧杆菌（BL）给药对实验性短肠综合征（SBS）BT的可能有益作用。方法：将128只成年Wistar大鼠在单独的代谢笼中保持10天，并分为3组：对照组（n = 71）：未操作的动物; RES组（n = 39）：从Treitz角以外10 cm至盲肠上方10 cm处切除80%的肠道; RES-PRO组（n = 18）：在口胃插管后进行相同的切除并每日给予7 8 X 10（8）CFU B乳酸菌。实验结束后处死动物，取肠系膜淋巴结（MLN）、外周血和门静脉血标本进行培养。血液细菌鉴定采用常规方法，MLN培养阳性，生长超过100 CFU/g。结果：对照组大鼠细菌移位率为6%。RES组BT的发生率为87%（39只动物中的34只），而RES-PRO组只有50%（18只动物中的9只）发生BT（P <0.05）。相对风险降低（RRR）为0.43（95%CI 0.14 - 0.72），需要治疗的人数（NNT）为3（95%CI 2 - 8）。换句话说，接受BL的动物的BT风险降低了43%（RRR为0.43），并且每3只接受治疗的动物中，预计有1只动物没有BT（NNT为3）。结论：在80%肠切除后，给予8个乳酸菌可降低成年Wistar大鼠的BT发生率。版权所有2002，爱思唯尔科学（美国）。All rights reserved.
Background/Purpose: Probiotics are live organisms that survive passage through the gastrointestinal tract and have beneficial effects on the host. Lactobacillus and Bifidobacterium have been recommended for cholesterol lowering, acute diarrhea, prevention of cancer, or inflammatory bowel disease. On the other hand, after massive bowel resection, bacterial overgrowth is frequent and favors bacterial translocation (BT). The possible beneficial effects of Bifidobacterium lactis (BL) administration on BT in experimental short bowel syndrome (SBS), have not been investigated. The aim of this study was to test the hypothesis that BL administration decreases BT in SBS in animals fed orally.Methods: One hundred twenty-eight adult Wistar rats fed orally with standard rat chow and tap water "ad libitum" were maintained in individual metabolic cages for 10 days and divided into 3 groups: control group (n = 71): nonmanipulated animals; RES group (n = 39): 80% gut resection from 10 cm beyond the angle of Treitz to 10 cm above the cecum; RES-PRO group (n = 18): same resection and daily 7 8 X 10(8) CFU B Lactis administration, after orogastric intubation. At the end of the experiment they were killed, and mesenteric lymph nodes (MLN) and peripheral and portal blood specimens were recovered and cultured. Bacterial identification in blood was made by conventional methods, and MLN culture was considered positive with a growth over 100 CFU/g.Results: Bacterial translocation was detected in 6% of control group rats. The incidence of BT In the RES group was 87% (34 of 39), whereas only 50% (9 of 18) of RES-PRO animals had BT (P < .05). The relative risk reduction (RRR) was 0.43 (95% CI 0.14 to 0.72), and the number needed to treat (NNT) was 3 (95% CI 2 to 8). In other words, animals that received BL had the risk of BT reduced by 43% (RRR of 0.43), and of every 3 animals treated, 1 is expected to be free of BT (NNT of 3).Conclusion: Administration of 8 Lactis reduces the incidence of BT in adult Wistar rats after 80% gut resection. Copyright 2002, Elsevier Science (USA). All rights reserved.