The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum
The Functional Quality of Soluble Recombinant Polypeptides Produced in Escherichia coli Is Defined by a Wide Conformational Spectrum
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DOI：
10.1128/aem.01446-08
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Villaverde, Antonio
中科院分区：
文献类型：
--
作者：
Martinez-Alonso, Monica;Gonzalez-Montalban, Nuria;Villaverde, Antonio
我们已经观察到，在大肠杆菌中产生的可溶性重组绿色荧光蛋白存在于宽的构象谱中。这导致不同的荧光蛋白组分，其中形态多样的可溶性聚集体丰富。因此，易聚集重组蛋白的可溶性版本的功能质量是统计学定义的，而不是由典型的天然结构的流行。
We have observed that a soluble recombinant green fluorescent protein produced in Escherichia coli occurs in a wide conformational spectrum. This results in differently fluorescent protein fractions in which morphologically diverse soluble aggregates abound. Therefore, the functional quality of soluble versions of aggregation-prone recombinant proteins is defined statistically rather than by the prevalence of a canonical native structure.