Genome-wide analysis of homeobox genes from Mesobuthus martensii reveals Hox gene duplication in scorpions
Genome-wide analysis of homeobox genes from Mesobuthus martensii reveals Hox gene duplication in scorpions
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DOI：
10.1016/j.ibmb.2015.04.002
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发表时间：
2015-06-01
影响因子：
3.8
通讯作者：
Cao, Zhijian
中科院分区：
文献类型：
--
作者：
Di, Zhiyong;Yu, Yao;Cao, Zhijian
同源盒基因属于一个大的基因群，它编码著名的dna结合同源结构域，在动物胚胎发育和细胞分化过程中起着关键作用。在此基础上，对亚洲蝎子Mesobuthus martensii （Chelicerata: Arachnida: Scorpiones: Buthidae）基因组序列进行了分析，共鉴定出149个同源盒基因。共鉴定出8类82个家族，其中ANTP类基因67个，PRD类基因33个，LIM类基因11个，POU类基因5个，SINE类基因6个，TALE类基因14个，CUT类基因5个，ZF类基因2个，未分类基因6个。转录组数据证实，超过一半的基因在成年人中表达。8个纲的同源盒基因多样性与先前分析的下颌骨节肢动物相似。有趣的是，据推测马氏蝎子可能有两个Hox星团。首次对螯合动物同源盒基因进行全基因组分析，不仅揭示了蝎类、蛛形动物和螯合动物同源盒基因的全库，而且对节肢动物同源盒基因的进化也有一定的启示。(C) 2015 Elsevier Ltd.版权所有。
Homeobox genes belong to a large gene group, which encodes the famous DNA-binding homeodomain that plays a key role in development and cellular differentiation during embryogenesis in animals. Here, one hundred forty-nine homeobox genes were identified from the Asian scorpion, Mesobuthus martensii (Chelicerata: Arachnida: Scorpiones: Buthidae) based on our newly assembled genome sequence with approximately 248 x coverage. The identified homeobox genes were categorized into eight classes including 82 families: 67 ANTP class genes, 33 PRD genes, 11 LIM genes, five POU genes, six SINE genes, 14 TALE genes, five CUT genes, two ZF genes and six unclassified genes. Transcriptome data confirmed that more than half of the genes were expressed in adults. The homeobox gene diversity of the eight classes is similar to the previously analyzed Mandibulata arthropods. Interestingly, it is hypothesized that the scorpion M. martensii may have two Hox clusters. The first complete genome-wide analysis of homeobox genes in Chelicerata not only reveals the repertoire of scorpion, arachnid and chelicerate homeobox genes, but also shows some insights into the evolution of arthropod homeobox genes. (C) 2015 Elsevier Ltd. All rights reserved.