Gene expression in menisci from the knees of skeletally immature and mature female rabbits
Gene expression in menisci from the knees of skeletally immature and mature female rabbits
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DOI：
10.1002/jor.1100170518
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发表时间：
1999-09-01
影响因子：
2.8
通讯作者：
Hart, DA
中科院分区：
文献类型：
--
作者：
Le Graverand, MPH;Reno, C;Hart, DA
本研究采用半定量逆转录-聚合酶链反应的敏感分子技术，评估了几种分子的mRNA水平，从内侧和外侧踝关节的成熟和未成熟的兔子的膝盖。采用TRIspin法从新西兰白色兔的内、外侧髓中提取总RNA。未成熟兔和成熟兔的两个脑髓中总RNA和DNA相似。总RNA进行逆转录和半定量聚合酶链反应分析，使用兔特异性引物集; mRNA水平的一个子集的分子不同的内侧和外侧的骨关节炎，这些变化的mRNA水平也受到影响的程度骨骼成熟的兔子。对于大多数的基因，mRNA水平一般高于在内侧半月板比在外侧半月板。来自未成熟和成熟兔的内侧半月板的分子如转化生长因子-β、环氧合酶-2和金属蛋白酶-1的组织抑制剂的mRNA水平显著增加。与此相反，与外侧半月板的mRNA相比，II型和III型胶原蛋白，双糖链蛋白聚糖，胰岛素样生长因子-2，纤溶酶原激活物抑制剂-1，基质金属蛋白酶-1在成熟兔的内侧半月板中显着增加，而多功能蛋白聚糖和I型胶原蛋白在未成熟兔的内侧半月板中显着增加。诱导型一氧化氮合酶和碱性成纤维细胞生长因子的mRNA水平在两个年龄组的两个tumerci相似。目前的研究表明，在内侧和外侧骨的mRNA水平的调节是组织特异性的，并受骨骼成熟的动物。
This study, using the sensitive molecular technique of semiquantitative reverse transcription-polymerase chain reaction, evaluated mRNA levels for several molecules in medial and lateral menisci from the knees of skeletally mature and immature rabbits. Total RNA was extracted from the medial and lateral menisci of New Zealand White rabbits with the TRIspin method. Total RNA and DNA were similar in the two menisci of both immature and mature rabbits. The total RNA was reverse-transcribed and analyzed by semiquantitative polymerase chain reaction using rabbit-specific primer sets; levels of mRNA for a subset of molecules differed between the medial and lateral menisci, These variations in mRNA levels were also influenced by the degree of skeletal maturity of the rabbits. For most of the genes, mRNA levels were generally higher in the medial than in the lateral meniscus. The medial meniscus from immature and mature rabbits had significantly increased levels of mRNA for molecules such as transforming growth factor-beta, cyclooxygenase-2, and tissue inhibitor of metalloprotease-l. In contrast, compared with mRNA in the lateral meniscus, that for types II and III collagen, biglycan, insulin-like growth factor-2, plasminogen activator inhibitor-1, and matrix metalloprotease-1 was significantly increased in the medial meniscus of mature rabbits only and that for versican and type-I collagen was significantly increased in the medial meniscus of immature rabbits only. Levels of mRNA for inducible nitric oxide synthase and basic fibroblast growth factor were similar in both menisci for both age groups. The present study demonstrates that regulation of mRNA levels in medial and lateral menisci is tissue-specific and influenced by the skeletal maturity of the animals.