Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation.
Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation.
复制标题
用氚化棕榈酸酯对 Ras 进行代谢标记，以监测棕榈酰化和去棕榈酰化。
DOI：
10.1007/978-1-62703-791-4_3
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Philips, Mark R
中科院分区：
文献类型：
--
作者：
Tsai, Frederick D;Wynne, Joseph P;Ahearn, Ian M;Philips, Mark R
用氚标记的棕榈酸酯进行代谢标记是一种直接监测这种脂肪酸对Ras蛋白的翻译后修饰的方法。增感屏的进步使氚的可视化变得容易，而不需要延长曝光时间。虽然更高能量的放射性同位素更容易可视化，但缺乏商业来源和需要屏蔽使它们更难以使用。由于放射性标记的棕榈酸酯直接掺入Ras中，因此可以通过传统的脉冲追踪实验来监测其损失，而传统的脉冲追踪实验无法通过酰基交换化学方法完成。因此，氚化棕榈酸酯仍然是一种容易获得的和直接的方法，用于监测在多种条件下Ras蛋白的棕榈酰化状态。
Metabolic labeling with tritiated palmitate is a direct method for monitoring post-translational modification of Ras proteins with this fatty acid. Advances in intensifying screens have allowed for the easy visualization of tritium without the need for extended exposure times. While more energetic radioisotopes are easier to visualize, the lack of commercial source and need for shielding make them more difficult to work with. Since radiolabeled palmitate is directly incorporated into Ras, its loss can be monitored by traditional pulse-chase experiments that cannot be accomplished with the method of acyl exchange chemistry. As such, tritiated palmitate remains a readily accessible and direct method for monitoring the palmitoylation status of Ras proteins under a multitude of conditions.