The CpxRA signal transduction system of Escherichia coli:: Growth-related autoactivation and control of unanticipated target operons
The CpxRA signal transduction system of Escherichia coli:: Growth-related autoactivation and control of unanticipated target operons
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DOI：
10.1128/jb.181.21.6772-6778.1999
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发表时间：
1999-11-01
影响因子：
3.2
通讯作者：
Lin, ECC
中科院分区：
文献类型：
--
作者：
De Wulf, P;Kwon, O;Lin, ECC
在大肠杆菌中，CpxRA 双组分信号转导系统感知细菌包膜中聚集和错误折叠的蛋白质并做出反应。我们发现 CpxR-P（同源反应调节剂的磷酸化形式）与 RpoS 一起激活 cpxRA 表达，表明 Cpx 系统参与稳定期生存。 CpxRA 系统参与蛋白质管理以外的功能，通过对 CpxR-P 识别共有序列进行基因组筛选后确定的几个假定目标来表明。通过电泳迁移率变动分析和 Northern 杂交，显示了 CpxR-P 对新鉴定的靶标 motABcheAW（指定运动性和趋化性）和 tsr（编码丝氨酸化学感受器）的直接阴性控制。结果表明，CpxRA 系统在广泛的应激反应网络中发挥着核心作用，其中蛋白质更新和能量保存的协调可能是统一的元素。
In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of the Cpx system in stationary-phase survival. Engagement of the CpxRA system in functions beyond protein management is indicated by several putative targets identified after a genomic screening for the CpxR-P recognition consensus sequence. Direct negative control of the newly identified targets motABcheAW (specifying motility and chemotaxis) and tsr (encoding the serine chemoreceptor) by CpxR-P was shown by electrophoretic mobility shift analysis and Northern hybridization. The results suggest that the CpxRA system plays a core role in an extensive stress response network in which the coordination of protein turnover and energy conservation may be the unifying element.