CHARACTERIZATION OF SILKWORM CHLOROPHYLL METABOLITES AS AN ACTIVE PHOTOSENSITIZER FOR PHOTODYNAMIC THERAPY
CHARACTERIZATION OF SILKWORM CHLOROPHYLL METABOLITES AS AN ACTIVE PHOTOSENSITIZER FOR PHOTODYNAMIC THERAPY
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DOI：
10.1021/np50087a010
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发表时间：
1992-09-01
影响因子：
5.1
通讯作者：
SONG, PS
中科院分区：
文献类型：
--
作者：
DAI, R;SHOEMAKER, R;SONG, PS
在远东地区，含有叶绿素代谢物的家蚕排泄物长期以来一直被用于药用活动。家蚕排泄物Me2CO提取物中的主要叶绿素衍生物(CPD)组分在体外对小鼠和人肿瘤细胞具有很强的光动力作用。分离、纯化了CPD的主要成分，并对其进行了广泛的结构和表征研究，以评估其光动力治疗活性。根据光谱分析，确定了主要未知化合物的结构为10-羟基叶绿酸a[1]。CPD组分对单线态分子氧的光敏化生产具有很高的量子效率(50%)，为其光动力学治疗效率提供了分子基础。另一个优点是，从这种CPD得到的‘’光氧化/降解‘’产物(S)保留了光动力增感剂的所有特性。
Silkworm excreta containing chlorophyll metabolites have long been used for their medicinal activities in the Far East. The major chlorophyll derivative (CpD) fraction in the Me2CO extract from the silkworm excreta exhibits strong photodynamic action on mice and human rumor cells in vitro. The main CpD component was isolated, purified, and subjected to extensive structural and characterization studies to assess its photodynamic therapeutic activity. According to the spectral analyses, the structure of the major unknown CpD was determined to be 10-hydroxypheophytin a[1]. The CpD component possessed a high quantum efficiency (50%) for the photosensitized production of singlet molecular oxygen, thus providing a molecular basis for its photodynamic therapeutic efficiency. A further advantage is that the ''photooxidation/degradation'' product(s) from this CpD retains all the characteristics of a photodynamic sensitizer.