Electron capture dissociation in a radio frequency ion trap
Electron capture dissociation in a radio frequency ion trap
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DOI：
10.1021/ac049309h
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发表时间：
2004-08-01
影响因子：
7.4
通讯作者：
Waki, I
中科院分区：
文献类型：
--
作者：
Baba, T;Hashimoto, Y;Waki, I
我们报告的第一个证据，电子捕获解离（ECD）在射频（RF）离子阱。肽离子，[物质P]（2+），捕获在一个二维的，线性射频离子阱被裂解的电子注入沿沿着中心轴的陷阱。沿着轴，磁场分量为零，施加50 mT的磁场。Ibis电子注入方案使电子的能量保持在1eV以下，防止它们被射频场加热。在0.2 μ A的电子流照射80 ms的条件下，目前的ECD效率接近4%。射频阱中的ECD可能会打开高通量和低成本的ECD应用，以获得与碰撞诱导解离互补的分子结构信息。
We report on the first evidence of electron capture dissociation (ECD) in a radio frequency (rf) ion trap. Peptide ions, [substance P](2+), trapped in a two-dimensional, linear rf ion trap were cleaved by electrons injected along the central axis of the trap. Along the axis, the if field component was zero and a magnetic field of 50 mT was applied. Ibis electron injection scheme keeps the energy of the electrons below 1 eV, preventing them from heating by the rf field. The present ECD efficiency is similar to4% by irradiation of electron current of 0.2 muA for 80 ms. ECD in rf traps may open high-throughput and low-cost ECD applications to obtain molecular structure information complementary to collision-induced dissociation.