QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREON‐12 1
QUICK FREEZING OF THE MURINE CNS: COMPARISON OF REGIONAL COOLING RATES AND METABOLITE LEVELS WHEN USING LIQUJD NITROGEN OR FREON‐12 1
复制标题
小鼠中枢神经系统的快速冷冻：使用液氮或氟利昂 12 时区域冷却速率和代谢物水平的比较 1
DOI：
10.1111/j.1471-4159.1972.tb01418.x
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发表时间：
1972
影响因子：
4.7
通讯作者：
M. M. Chang
中科院分区：
文献类型：
--
作者：
J. Ferrendelli;M. H. Gay;W. G. Sedgwick;M. M. Chang
摘要-将小鼠大脑在液氮或冷却至冰点的氟利昂-12中原位冷冻。这些冷却剂的冷却速度和时间在不同的中枢神经系统区域的影响进行了测定。此外，测量了在任一冷却剂中冷冻的完整动物和切断的头部的脑的选定区域中的ATP、P-肌酸和乳酸盐的水平。对于完整的动物和切断的头部，大脑的浅表区域冷却到0°C，深层区域冷却到25°C，在液氮或氟利昂中的速度相同。随后的冷却更迅速的液氮在这两个区域。在两种冷却剂中冷冻的大脑中，ATP、P-肌酸和乳酸的水平相似，这可能是因为随着体温下降，CNS对高能磷酸盐的利用显著下降。在冷冻完整的动物大脑中，ATP和P-肌酸水平高于冷冻前切断的头部大脑，乳酸水平低于冷冻前切断的头部大脑。这种差异可能是伴随断头的显著刺激的结果，也可能反映了将动物浸入冷却剂后，完好动物短暂持续的脑循环。
Abstract— Murine brains were frozen in situ, either in liquid N2 or in Freon‐12 cooled to its freezing point. The effect of these coolants on cooling rates and times in various CNS regions was determined. In addition, levels of ATP, P‐creatine and lactate were measured in selected regions of brains from both intact animals and severed heads frozen in either coolant. For both the intact animals and severed heads, superficial regions of brain cooled to 0°C and deeper regions to 25°C, at the same rate in either liquid N2 or Freon. Subsequent cooling was more rapid in liquid N2 in both regions. Levels of ATP, P‐creatine and lactate were similar in brains frozen in either coolant, probably because CNS utilization of highenergy phosphates decreased markedly as body temperature fell. In brains of animals frozen intact, levels of ATP and P‐creatine were higher and levels of lactate were lower than those in brains from heads which were severed prior to freezing. This difference may be a result of the marked stimulation which accompanies decapitation and may also reflect continued cerebral circulation in the intact animal for a brief time after immersing the animal in the coolant.