Tri-dimensional morphometric analysis of astrocytic processes with high voltage electron microscopy of thick Golgi preparations
Tri-dimensional morphometric analysis of astrocytic processes with high voltage electron microscopy of thick Golgi preparations
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DOI：
10.1023/b:neur.0000044189.08240.a2
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发表时间：
2004-05-01
期刊：
影响因子：
--
通讯作者：
Ellisman, MH
中科院分区：
文献类型：
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作者：
Hama, K;Arii, T;Ellisman, MH
星形胶质细胞过程的一个特征是呈现其他大脑成分的胫骨片或片状覆盖物的形式。我们通过计算机电子断层扫描和立体摄影测量，使用厚高尔基体制备物的倾斜高压电子显微镜图像，分析了大鼠齿状回分子层中原生质星形胶质细胞的广泛和精细划分的过程。测量星形胶质细胞过程的表面积和体积并估计表面/体积比。神经丛中星形细胞突起的表面/体积比范围为每妈妈 18.9 至 33.0，平均值为每妈妈 26.2 +/- 5.0。这些值与之前报道的大鼠小脑伯格曼神经胶质细胞末端突起微区的值大致相当，这些值是根据薄连续切片电子显微镜图像的重建估计的。神经丛中星形胶质细胞突起的大表面与体积比是由于插入其他细胞元件之间的突起的层状性质造成的，并且可能反映了星形胶质细胞的功能活动。结果表明，电子断层扫描和立体摄影测量对于星形胶质细胞过程的三维形态测量分析是有用的，尽管这两种技术都有望进一步完善，以便为这些复杂的过程提供更精确的测量。
A characteristic feature of the astrocytic processes is to assume the form of shin sheets or lamellate coverings of other brain constituents. We analyzed the extensive and finely divided processes of the protoplasmic astrocyte in the molecular layer of the rat dentate gyrus by means of computer electron tomography and stereo-photogrammetry using tilted high voltage electron microscope images of thick Golgi preparations. The surface area and volume of the astrocytic processes were measured and the surface/volume ratios were estimated. The surface/volume ratios of astrocytic processes in the neuropile ranged from 18.9 to 33.0 per mum, and the mean value was 26.2 +/- 5.0 per mum. The values were roughly comparable to those previously reported for the microdomain of Bergmann glia cell terminal processes in the rat cerebellum, which were estimated from reconstructions using thin serial section electron microscope images. The large surface to volume ratio of the astrocytic processes in the neuropile resulted from the lamellar nature of the processes interposed between other cellular elements, and may reflect the functional activities of the astrocyte. The results suggest the usefulness of the electron tomography and stereo-photogrammetry for three-dimensional morphometrical analysis of the astrocytic processes, although both techniques can be expected to be refined further in order to provide more precise measurements of these complicated processes.