Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot™ MTA and White MTA
Osteoblasts and MG-63 osteosarcoma cells behave differently when in contact with ProRoot™ MTA and White MTA
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DOI：
10.1046/j.1365-2591.2003.00691.x
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发表时间：
2003-08-01
影响因子：
5
通讯作者：
Opperman, LA
中科院分区：
文献类型：
--
作者：
Pérez, AL;Spears, R;Opperman, LA
目的验证MG-63成骨肉瘤细胞和原代成骨细胞对ProRoot（TM）MTA的不同反应（i）研究原代成骨细胞和MG-63骨肉瘤细胞对ProRootTM NITA和白色MTA的附着;以及（ii）比较两种细胞系与这些牙髓材料接触的成骨行为。方法从胎鼠中收获原代成骨细胞通过顺序消化获得颅骨，并购买MG-63骨肉瘤细胞。将细胞暴露于根据制造商的说明书制备的ProRootTM MTA和白色MTA。所有样品和对照品均以四次重复制备。在暴露于MTA 6、9和13天后，将细胞固定并准备用于SEM检查。此外，两种细胞类型生长至汇合，并暴露于β-甘油磷酸盐和地塞米松，以评估矿化结节形成作为成骨行为的函数。除了白色NITA之外，在13天结束时在材料的顶部上看不到初级成骨细胞。暴露后的细胞分化培养基结节中观察到的培养的原代成骨细胞，但不是MG-63骨肉瘤cells.Conclusions在本研究的条件下，而原代成骨细胞最初绑定到白色NITA，他们没有生存的表面上结束的13天。当暴露于分化培养基时，原代成骨细胞形成矿化结节，而MG-63细胞不形成结节。因为MG-63细胞不通过形成矿化结节表现成骨。原代成骨细胞比MG-63骨肉瘤细胞对白色NITA更敏感。原代成骨细胞比MG-63细胞更适合于在细胞培养中测试牙髓材料。
Aim To test the hypothesis that MG-63 osteosarcoma cells and primary osteoblasts react differently to ProRoot(TM) MTA (mineral trioxide aggregate) and White MTA by: (i) investigating the attachment of primary osteoblasts and MG-63 osteosarcoma cells to ProRoot(TM) NITA and White MTA; and (ii) comparing the osteogenic behaviour of both cell lines in contact with these endodontic materials.Methodology Primary osteoblasts were harvested from foetal rat calvaria by sequential digestion and MG-63 osteosarcoma cells were purchased. Cells were exposed to ProRoot(TM) MTA and White MTA prepared according to the manufacturer's instructions. All samples and controls were prepared in quadruplicate. After 6, 9 and 13 days exposure to MTA, the cells were fixed and prepared for SEM examination. In addition, both the cell types were grown to confluence and exposed to beta-glycerophosphate and dexamethasone to assess mineralized nodule formation as a function of osteogenic behaviour.Results The number of cells on the surface of the culture dish and on top of the materials increased mall samples throughout the 3 time periods, except for White NITA where no primary osteoblasts were visible on top of the material by the end of 13 days. After exposing cells to differentiation medium nodules were observed in cultures of primary osteoblasts, but not of MG-63 osteosarcoma cells.Conclusions Under the conditions of this study, whilst primary osteoblasts initially bound to White NITA, they did not survive on the surface by the end of 13 days. Primary osteoblasts formed mineralized nodules when exposed to differentiation medium, whilst MG-63 cells did not form nodules. As MG-63 cells do not behave osteogenically by forming mineralized nodules. and primary osteoblasts are more sensitive than MG-63 osteosarcoma cells to White NITA in cell culture. primary osteoblasts are more appropriate than MG-63 cells for testing endodontic materials in cell culture.