Metabolomic analysis reveals the potential metabolites and pathogenesis involved in mulberry yellow dwarf disease
Metabolomic analysis reveals the potential metabolites and pathogenesis involved in mulberry yellow dwarf disease
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代谢组学分析揭示桑树黄矮病的潜在代谢物和发病机制
DOI：
10.1111/pce.12255
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发表时间：
2014-06-01
影响因子：
7.3
通讯作者：
Ji, Xian-Ling
中科院分区：
文献类型：
--
作者：
Gai, Ying-Ping;Han, Xue-Juan;Ji, Xian-Ling
为探讨植原体致病的分子机制，采用气相色谱-质谱联用技术研究了植原体侵染后桑葚和韧皮部汁液的综合代谢组学变化。叶片和韧皮部汁液的代谢产物组成不同，植原体感染对韧皮部汁液的代谢产物组的影响大于叶片。植原体侵染引起了叶片中碳水化合物、氨基酸、有机酸等代谢产物含量的变化，同时生化分析结果表明，淀粉的降解受到抑制，侵染叶片中淀粉含量增加。此外，我们还发现植原体感染改变了脱落酸和细胞分裂素的水平，打破了植物激素的平衡。感兴趣的是，我们的数据表明，H_2O_2和超氧阴离子的含量增加，在感染的叶片，但不是在韧皮部汁液。基于这些结果，我们检测了一些代谢物变化的代谢相关基因的表达水平，并讨论了这些变化的潜在分子机制。结果表明，桑树叶片和韧皮部汁液对植原体侵染的代谢反应复杂，但反应机制不同;利用GC/MS技术对桑树叶片和韧皮部汁液的多靶点代谢产物进行了分析，首次从整体上研究了桑葚感染黄矮病植原体后的代谢变化。结合整体代谢物分析，分析了一些基因表达和生化变化，并讨论了这些变化的潜在分子机制。指出植物叶片和韧皮部汁液对植原体侵染都有复杂的代谢反应，但它们的代谢变化和反应机制不同。
To analyse the molecular mechanisms of phytoplasma pathogenicity, the comprehensive metabolomic changes of mulberry leaf and phloem sap in response to phytoplasma infection were examined using gas chromatography-mass spectrometry. The metabolic profiles obtained revealed that the metabolite compositions of leaf and phloem sap were different, and phytoplasma infection has a greater impact on the metabolome of phloem sap than of leaf. Phytoplasma infection brought about the content changes in various metabolites, such as carbohydrates, amino acids, organic acids, etc. Meanwhile, the results of biochemical analysis showed that the degradation of starch was repressed, and the starch content was increased in the infected leaves. In addition, we found that phytoplasma infection changed the levels of abscisic acid and cytokinin and break phytohormone balance. Interestingly, our data showed that the contents of H2O2 and superoxide were increased in the infected leaves, but not in the phloem saps. Based on the results, the expression levels of the genes involved in the metabolism of some changed metabolites were examined, and the potential molecular mechanisms of these changes were discussed. It can be concluded that both the leaf and phloem saps have a complicated metabolic response to phytoplasma infection, but their response mechanisms were different.A multi target metabolite profiling was characterized by GC/MS to provide for the first time a global analysis of the changes that occur in mulberry following infection by yellows dwarf phytoplasma. Together with global metabolite analysis, some gene expressions and biochemical changes were analyzed, and the potential molecular mechanisms of these changes were discussed. It was pointed out that both the leaf and phloem sap have a complicated metabolic response to phytoplasma infection, but their metabolic changes and response mechanisms were different.