The effect of buttermilk consumption on biofilm formation on silicone rubber voice prostheses in an artificial throat
The effect of buttermilk consumption on biofilm formation on silicone rubber voice prostheses in an artificial throat
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酪乳消耗对人工喉咙中硅橡胶发声器生物膜形成的影响
DOI：
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发表时间：
1998
影响因子：
2.6
通讯作者：
F. Albers
中科院分区：
文献类型：
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作者：
H. Busscher;G. M. Bruinsma;R. Weissenbruch;C. Leunisse;H. C. Mei;F. Dijk;F. Albers
摘要喉切除术后留置硅橡胶发音假体上生物膜的形成仍然是导致瓣膜功能障碍的主要原因，从而导致频繁的置换。在荷兰的患者支持团体中，喉切除者建议，食用酪乳可以延长留置硅橡胶发音假体的寿命。本研究的目的是比较格罗宁根纽扣发音假体在所谓的人工喉中的生物膜形成。将10个假体置于模拟对照组，另10个假体置于模拟酪乳组，每天3次，每次700ml。通过将从移植的格罗宁根按钮语音假体中分离出来的全部可培养微生物(细菌和酵母菌)接种到两个人造喉咙中，允许生物膜在假体上生长。3天后，1只人工喉每日3次注入磷酸盐缓冲液(对照组)，8天后另一只人工喉注入酪乳液。对照组从人工喉取出的假体被厚厚的生物膜覆盖。扫描电子显微镜显示硅橡胶中长出了微菌落，类似于在解释的格罗宁根纽扣上观察到的内生。在实验期间，在另一个人造喉咙中模拟酪乳的消耗几乎完全阻止了假体上生物膜的形成。这些在人工喉中的体外实验表明，每天摄入酪乳可以通过抑制生物膜的形成来减轻发音假体的恶化。
Abstract Biofilm formation on indwelling silicone rubber voice prostheses in laryngectomized patients is still the main reason for dysfunction of the valve, leading to frequent replacements. Within patient support groups in The Netherlands, laryngectomees have suggested that the consumption of buttermilk prolongs the life-time of indwelling silicone rubber voice prostheses. The aim of the present study was to compare biofilm formation on Groningen button voice prostheses in a so-called artificial throat. Ten prostheses were placed in a simulated control group and ten other prostheses in a group with a simulated consumption of 700 ml buttermilk three times a day. Biofilms were allowed to grow on the prostheses by inoculating two artificial throats with the total cultivable microflora (bacteria and yeasts) isolated from an explanted Groningen button voice prosthesis. After 3 days, one artificial throat was perfused three times daily with phosphate buffer (control group) for 8 days, while the other artificial throat was perfused with buttermilk. Prostheses removed from the artificial throat in the control group were covered with a thick biofilm. Scanning electron microscopy showed microcolonies growing into the silicone rubber, similar to the ingrowth observed on explanted Groningen buttons. The simulated consumption of buttermilk in the other artificial throat almost fully prevented the formation of a biofilm on the prostheses during the experimental period. These in vitro experiments in the artificial throat demonstrate that the deterioration of voice prostheses can be lessened by the daily intake of buttermilk through its inhibitory effects on biofilm formation.