Enhancement of the endosomal endocytic pathway increases quantal size
Enhancement of the endosomal endocytic pathway increases quantal size
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DOI：
10.1016/j.mcn.2008.10.005
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发表时间：
2009-02-01
影响因子：
3.5
通讯作者：
Bykhovskaia, Maria
中科院分区：
文献类型：
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作者：
Akbergenova, Yulia;Bykhovskaia, Maria
我们结合自发量子事件的记录与突触超微结构的电子显微镜分析，以证明神经分泌量子的大小增加激活后的内体内吞途径。我们可逆地激活内体内吞途径在果蝇运动终扣的应用高K+溶液。这种处理产生了许多池、空泡和扩大的小泡的形成。自发量子事件记录后立即停止高K+应用显着扩大，量子大小的增加后，10分钟的休息期被逆转。由latrunculin B预处理产生的肌动蛋白解聚抑制了内体样结构的形成和量子尺寸的增加。加载的制剂与染料FM 1 -43，然后通过光转化的染料结合电子显微镜分析显示，所观察到的池很可能是产品的散装膜检索和囊泡融合。(C)2008年爱思唯尔公司All rights reserved.
We combined recordings of spontaneous quantal events with electron microscopy analysis of synaptic ultrastructure to demonstrate that the size of a neurosecretory quantum increases following an activation of the endosomal endocytic pathway. We reversibly activated the endosomal endocytic pathway in Drosophila motor boutons by application of high K+ solution. This treatment produced the formation of numerous cisternae, vacuoles and enlarged vesicles. Spontaneous quantal events recorded immediately after the cessation of high K+ application were significantly enlarged, and this increase in quantal size was reversed after a 10 minute resting period. Actin depolymerization produced by latrunculin B pretreatment inhibited both the formation of endosome-like structures and the increase in quantal size. Loading the preparations with the dye FM1-43 followed by photoconversion of the dye combined with electron microscopy analysis revealed that the observed cisternae are likely to be the product of both bulk membrane retrieval and vesicle fusion. (C) 2008 Elsevier Inc. All rights reserved.