Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney
Crystallization and preliminary crystallographic analysis of D-aspartate oxidase from porcine kidney
复制标题
猪肾D-天冬氨酸氧化酶的结晶及初步晶体学分析
DOI：
10.1107/s1744309112013243
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Senda T.
中科院分区：
文献类型：
--
作者：
Senda M.;Yamamoto A.;Tanaka H.;Ishida T.;Horiike K.;Senda T.
以PEG 8000为沉淀剂，采用坐滴气相扩散法从猪肾中结晶出d-天冬氨酸氧化酶（DDO）。晶体属P21空间群，晶胞参数a = 79.38，B = 144.0，c = 80.46 nm，β = 101.1°，衍射分辨率为1.80 nm。 使用与DDO序列相同42%的人d-氨基酸氧化酶结构作为搜索模型的分子替代试验提供了令人满意的解决方案。
d-Aspartate oxidase (DDO) from porcine kidney was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as a precipitant. The crystal belonged to space group P21, with unit-cell parameters a = 79.38, b = 144.0, c = 80.46 Å, β = 101.1°, and diffracted to 1.80 Å resolution. Molecular-replacement trials using the structure of human d-amino-acid oxidase, which is 42% identical in sequence to DDO, as a search model provided a satisfactory solution.