Apomorphine-induced modulation of neural activities in the ventrolateral striatum of rats
Apomorphine-induced modulation of neural activities in the ventrolateral striatum of rats
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DOI：
10.1002/syn.21644
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发表时间：
2013-07-01
期刊：
影响因子：
2.3
通讯作者：
Kobayashi, Masayuki
中科院分区：
文献类型：
--
作者：
Fujita, Satoshi;Kato, Risako;Kobayashi, Masayuki
纹状体腹外侧部的多巴胺能系统(SVL)是基底节的一部分，调节口面部运动；双侧共同刺激多巴胺D_1和D_2样受体可引起大鼠重复的颌骨运动。然而，多巴胺能受体的激活如何调节SVL神经元的活动仍不清楚。我们系统地注射阿朴吗啡，一种非选择性的多巴胺受体激动剂，在乌拉坦麻醉下诱导颌骨运动，并从SVL神经元进行多通道单位记录。SVL神经元分为两类：(1)以中等棘神经元和部分GABA能中间神经元为代表的相活动(PA)神经元；(2)以胆碱能中间神经元为主的强直活动(TA)神经元。阿朴吗啡可调节PA神经元的放电频率，易化率为33.3%，抑制率为38.3%。阿朴吗啡可使大部分TA神经元的放电频率降低(71.1%)。分析了PA和PA、PA和TA以及TA和TA神经元之间的相互关系，发现对PA神经元和PA和TA神经元对阿朴吗啡诱导的同步化棘波数目的影响可以忽略不计。相反，TA神经元之间的配对显示同步棘波的数量持续减少。阿朴吗啡对TA神经元活动的抑制与同步化输出的减少可能减少了局部释放的乙酰胆碱，这可能是阿朴吗啡诱导大鼠下颌运动的原因之一。Synapse 67：363373,2013年。(C)2013年威利期刊公司。
The dopaminergic system in the ventrolateral portion of the striatum (Svl), part of the basal ganglia, regulates orofacial movements; bilateral co-stimulation of both dopamine D1-like and D2-like receptors elicits repetitive jaw movements in rats. However, how the activities of Svl neurons are modulated by the activation of dopaminergic receptors remains unknown. We systematically injected apomorphine, a non-selective dopamine receptor agonist that induced jaw movements under urethane anesthesia, and performed multi-channel unit recording from Svl neurons. The Svl neurons were classified into two subgroups: (1) the phasically active (PA) neurons represented by mainly the medium spiny neurons and the GABAergic interneurons in part, and (2) the tonically active (TA) neurons composed of mainly the cholinergic interneurons. Apomorphine modulated PA neuron firing frequency with wide variability; 33.3% of the PA neurons were facilitated, while 38.3% were suppressed. In the majority of TA neurons, the firing frequency was reduced by apomorphine (71.1%). The cross-correlations between PA and PA, PA and TA, and TA and TA neurons were analyzed, and pairs of PA neurons and pairs of PA and TA neurons, showed negligible apomorphine-induced effect on the number of synchronized spikes. In contrast, pairs between TA neurons showed a consistent decrease in the number of synchronized spikes. The apomorphine-induced suppression of TA neuron activities with decreased synchronized outputs is likely to reduce the amount of locally released acetylcholine, which may contribute to the induction of apomorphine-induced jaw movements in rats. Synapse 67:363373, 2013. (c) 2013 Wiley Periodicals, Inc.