Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations.
Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations.
复制标题
甘氨酸受体突变小鼠运动神经元的内在和突触稳态可塑性。
DOI：
10.1152/jn.00728.2013
复制
发表时间：
2014
影响因子：
2.5
通讯作者：
Callister,RJ
中科院分区：
文献类型：
--
作者：
Tadros,MA;Farrell,KE;Schofield,PR;Brichta,AM;Graham,BA;Fuglevand,AJ;Callister,RJ
抑制性突触输入到舌下运动神经元（HMs）对调节脑干回路的兴奋性是重要的。在这里，我们要问的是，在三种具有甘氨酸受体（GlyR）不同自然突变的小鼠突变体中，抑制降低是否会导致内在和/或突触稳态可塑性。从野生型（wt）、痉挛型（spd）、痉挛型（spa）和振荡型（ot）小鼠（C57Bl/6，大约出生后21天）的横切脑干切片的HMs中获得全细胞记录。被动电位和动作电位（AP）的性质与对照组相似。相比之下，spahm具有更低的输入电阻，更多的去极化静息膜电位，更高的流变基电流，更小的AP振幅，以及更慢的超极化后电流衰减时间。通过注射电流/发射频率图的“增益”来评估，所有品系的HMs兴奋性相似，而反弹尖峰的发生率随时间增加。在斜坡电流注入期间，AP放电的招聘和招聘电流（即ΔI）之间的差异更为负激励。gaba -氨基抑制性突触后电流（mIPSC）振幅在突变体中增加，但在突变体中没有增加，这表明甘氨酸能驱动的减少导致了这些突变体中其他主要的快速抑制性突触递质系统的代偿调节。总的来说，我们的数据表明，长期减少甘氨酸能驱动HMs导致内在和突触特性的变化，这与内稳态可塑性一致，但与内稳态可塑性不一致。我们提出这种可塑性是稳定HM输出的一种尝试，这种尝试成功或失败。
Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in three murine mutants with distinct naturally occurring mutations in the glycine receptor (GlyR), leads to intrinsic and/or synaptic homeostatic plasticity. Whole cell recordings were obtained from HMs in transverse brainstem slices from wild-type (wt), spasmodic (spd), spastic (spa), and oscillator (ot) mice (C57Bl/6, approximately postnatalday 21). Passive and action potential (AP) properties inspdandotHMs were similar towt. In contrast,spaHMs had lower input resistances, more depolarized resting membrane potentials, higher rheobase currents, smaller AP amplitudes, and slower afterhyperpolarization current decay times. The excitability of HMs, assessed by “gain” in injected current/firing-frequency plots, was similar in all strains whereas the incidence of rebound spiking was increased inspd. The difference between recruitment and derecruitment current (i.e., ΔI) for AP discharge during ramp current injection was more negative inspaandot. GABAAminiature inhibitory postsynaptic current (mIPSC) amplitude was increased inspaandotbut notspd, suggesting diminished glycinergic drive leads to compensatory adjustments in the other major fast inhibitory synaptic transmitter system in these mutants. Overall, our data suggest long-term reduction in glycinergic drive to HMs results in changes in intrinsic and synaptic properties that are consistent with homeostatic plasticity inspaandotbut not inspd. We propose such plasticity is an attempt to stabilize HM output, which succeeds inspabut fails inot.
登录
查看更多内容
发育期尼古丁暴露会改变舌下运动神经元的神经传递和兴奋性。
影响因子：
2.5
作者：
Pilarski,JasonQ;Wakefield,HilaryE;Fuglevand,AndrewJ;Levine,RichardB;Fregosi,RalphF
通讯作者：
Fregosi,RalphF
影响因子：
3.3
作者：
Camp, A. J.;Lim, R.;Brichta, A. M.
通讯作者：
Brichta, A. M.
影响因子：
5.5
作者：
BISCOE, TJ;DUCHEN, MR
通讯作者：
DUCHEN, MR
利用鼠突变体研究甘氨酸受体功能
DOI：
10.1046/j.1440-1681.1999.03148.x
发表时间：
1999
影响因子：
2.9
作者：
Rj Callister;P. Schofield;P. Sah
通讯作者：
P. Sah
痉挛小鼠甘氨酸受体α1亚基的点突变影响激动剂反应
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
B. Saul;V. Schmieden;C. Kling;C. Mülhardt;P. Gass;J. Kuhse;C. Becker
通讯作者：
C. Becker