THE GENE ENCODING CAMP RECEPTOR PROTEIN IS REQUIRED FOR COMPETENCE DEVELOPMENT IN HAEMOPHILUS-INFLUENZAE RD
THE GENE ENCODING CAMP RECEPTOR PROTEIN IS REQUIRED FOR COMPETENCE DEVELOPMENT IN HAEMOPHILUS-INFLUENZAE RD
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DOI：
10.1073/pnas.89.5.1626
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发表时间：
1992-03-01
影响因子：
11.1
通讯作者：
CHANDLER, MS
中科院分区：
文献类型：
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作者：
CHANDLER, MS
流感嗜血杆菌 Rd 菌株 JG87 含有单个微型 Tn1Okan 插入，导致遗传转化能力发育缺陷。对含有该插入突变的 DNA 片段以及野生型基因座进行了克隆、定位和测序。该序列包含 224 个氨基酸的蛋白质的开放阅读框，预测 M(r) 为 25,152。推导的蛋白质序列与大肠杆菌cAMP受体蛋白质具有很强的相似性。克隆在多拷贝质粒上的大肠杆菌 crp 基因被证明可以完全补充突变菌株的能力缺陷表型；因此，流感嗜血杆菌基因被命名为crp。这些结果表明，流感嗜血杆菌cAMP-cAMP受体蛋白复合物的功能是调节一个或多个对于流感嗜血杆菌Rd能力发展所必需的启动子。还描述了与大肠杆菌 ttk 基因同源的流感嗜血杆菌 crp 上游基因的特征。
The Haemophilus influenzae Rd strain JG87 contains a single mini-Tn1Okan insertion that causes a deficiency in the development of competence for genetic transformation. The DNA fragment containing this insertion mutation, as well as the wild-type locus, was cloned, mapped, and sequenced. The sequence contained an open reading frame for a protein of 224 amino acids with a predicted M(r) of 25,152. The deduced protein sequence showed strong similarity to the Escherichia coli cAMP receptor protein. The E. coli crp gene cloned on a multicopy plasmid was shown to fully complement the competence-deficient phenotype of the mutant strain; thus, the H. influenzae gene was named crp. These results suggest that H. influenzae cAMP-cAMP receptor protein complex functions to regulate one or more promoters essential for the development of competence in H. influenzae Rd. Features of a gene upstream of H. influenzae crp that is homologous to the E. coli ttk gene are also described.