Multichannel nanoplasmonic platform for imidacloprid and fipronil residues rapid screen detection
Multichannel nanoplasmonic platform for imidacloprid and fipronil residues rapid screen detection
复制标题
用于吡虫啉和氟虫腈残留快速筛查检测的多通道纳米等离子体平台
DOI：
10.1016/j.bios.2020.112677
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发表时间：
2020
影响因子：
12.6
通讯作者：
Wei Pei-Kuen
中科院分区：
文献类型：
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作者：
Wang Sheng-Hann;Lo Shu-Cheng;Tung Yung-Ju;Kuo Chia-Wen;Tai Yi-Hsin;Hsieh Shu-Yi;Lee Kuang-Li;Hsiao Shune-Rung;Sheen Jenn-Feng;Hsu Ju-Chun;Wei Pei-Kuen
近年来，据报道，吡虫啉和氟虫腈会危害蜜蜂等益虫，并对包括人类在内的哺乳动物构成潜在风险。考虑到它们的广泛使用和潜在的最低毒性范围从10 ppb到1 ppm（取决于物种），迫切需要一种简单，快速，灵敏和可靠的筛选和检测方法。在这里，我们提出了一种基于表面等离子体共振（SPR）的纳米等离子体芯片集成多通道光谱成像系统，以检测生态系统危害农药。巯基半抗原的预修饰使检测时间缩短至2.5 h。此外，由于多通道结构，可以引入内标分析方法，有效降低真实的样品中的基体干扰，从而更准确地测定目标农药的浓度。加标样品检测结果的强线性表明目标农药的定量具有较高的准确性。考虑到检测限（~10 ppb），将检测和定量的截止值分别设定为15和45 ppb，并用作检测标准。对真实的样品进行盲法检测，并与液相色谱电喷雾串联质谱法（标准方法）检测结果进行比较，结果高度一致。定制的集成SPR系统允许更简单，无标记，高通量，可靠的现场识别和定量的吡虫啉和氟虫腈。所有测试结果都验证了该平台在现场快速筛查和检测十亿分之一和百万分之一水平农药残留的能力。
In recent years, imidacloprid and fipronil have been reported to harm beneficial insects, such as honey bees, and to potentially pose risks to mammals, including humans. Considering their widespread use and potential minimum toxic range from 10 ppb to 1 ppm (species dependent), a simple, rapid, sensitive, and reliable method for screening and detection is urgently needed. Here, we present a surface plasmon resonance (SPR)-based nanoplasmonic chip integrated with a multichannel spectral imaging system to detect ecosystem-harming pesticides. The pre-modification of the designed mercapto-haptens reduced detection time to 2.5 h. Moreover, owing to the multichannel configuration, it was possible to introduce an internal standard analytical method to effectively reduce matrix interference in real samples; thus, the concentration of the target pesticide could be determined more precisely. The strong linearity of the spiked sample test results indicated high accuracy in quantifying target pesticides. Considering the limit of detection (~10 ppb), the cutoffs for detection and quantification were set at 15 and 45 ppb, respectively, and were used as the detection criteria. The detection results of the blind tests of real samples were also compared with those of liquid chromatography electrospray ionization tandem mass spectrometry (standard method) and were highly consistent. The custom-made integrated SPR system allows much simpler, label-free, high-throughput, and reliable on-site identification and quantification of imidacloprid and fipronil. All test results validated the platform's capability in the on-site rapid screening and detection of pesticide residues at the parts per billion and parts per million levels.