CHARACTERIZATION OF A MONOCLONAL ANTI-BETA-2-MICROGLOBULIN ANTIBODY AND ITS USE IN THE GENETIC AND BIOCHEMICAL-ANALYSIS OF MAJOR HISTOCOMPATIBILITY ANTIGENS
CHARACTERIZATION OF A MONOCLONAL ANTI-BETA-2-MICROGLOBULIN ANTIBODY AND ITS USE IN THE GENETIC AND BIOCHEMICAL-ANALYSIS OF MAJOR HISTOCOMPATIBILITY ANTIGENS
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DOI：
10.1002/eji.1830090709
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发表时间：
1979-01-01
影响因子：
5.4
通讯作者：
PARHAM, P
中科院分区：
文献类型：
--
作者：
BRODSKY, FM;BODMER, WF;PARHAM, P
通过小鼠骨髓瘤P3-X63-Ag 8和来自用Molt 4（一种人[肿瘤性] T [胸腺衍生的]细胞系）免疫的BALB/c小鼠的脾细胞之间的细胞融合来产生单克隆抗β 2-微球蛋白（BBM.1抗体）。BBM. 1抗体被可溶性β 2-微球蛋白和纯化的HLA-A、B抗原完全抑制，并且仅在人-小鼠体细胞杂交体具有15号染色体并表达人β 2-微球蛋白的情况下与人-小鼠体细胞杂交体反应。在补体依赖性溶解和IG[免疫球蛋白]G类中具有细胞毒性。1和单克隆抗HLA-A、B、C糖蛋白抗体W 6/32用于定量不同人细胞类型上β 2-微球蛋白和HLA-A、B、C糖蛋白的相对量。胸腺细胞和Molt 4细胞系显示β 2-微球蛋白比HLA-A、B、C糖蛋白显著过量，如通过W 6/32反应性所测量的。B [骨髓衍生]细胞系、外周血淋巴细胞、成纤维细胞、HeLa细胞衍生物和HSB-2（另一种T细胞系）的数量相等。用BBM. 1和W 6/32抗体检测HLA-A、B、C抗原和β 2-微球蛋白及其在其它物种中的同源物之间的免疫交叉反应。W 6/32抗原决定簇似乎比BBM.1抗体识别的抗原决定簇更高度保守。
A monoclonal anti-.beta.2-microglobulin (BBM.1 antibody) was produced by cell fusion between the mouse myeloma, P3-X63-Ag8 and spleen cells from a BALB/c mouse immunized with Molt 4, a human [neoplastic] T [thymus-derived] cell line. BBM.1 antibody was fully inhibited by soluble .beta.2-microglobulin and purified HLA-A, B antigens and reacted with human-mouse somatic cell hybrids only if they had chromosome 15 and expressed human .beta.2-microglobulin. It was cytotoxic in complement-dependent lysis and of the Ig[immunoglobulin]G class. BBM.1 and a monoclonal anti-HLA-A, B, C glycoprotein antibody, W6/32, were used to quantitate relative amounts of .beta.2-microglobulin and HLA-A, B, C glycoproteins on different human cell types. Thymocytes and the Molt 4 cell line showed a considerable excess of .beta.2-microglobulin over HLA-A, B, C glycoproteins, as measured by W6/32 reactivity. B [bone marrow-derived] cell lines, peripheral blood lymphocytes, fibroblasts, a HeLa cell derivative and HSB-2, another T cell line, had equal amounts. Immunological cross-reactions between HLA-A, B, C antigens and .beta.2-microglobulin and their homologs in other species were detected with the BBM.1 and W6/32 antibodies. The W6/32 antigenic determinant appeared to be more highly conserved than that recognized by the BBM.1 antibody.