Isobaric tags for relative and absolute quantitation-based quantitative proteomics analysis provides novel insights into the mechanism of cross-incompatibility between tree peony and herbaceous peony
Isobaric tags for relative and absolute quantitation-based quantitative proteomics analysis provides novel insights into the mechanism of cross-incompatibility between tree peony and herbaceous peony
复制标题
基于相对和绝对定量的定量蛋白质组学分析的同量异位标签为牡丹和草本牡丹之间的交叉不亲和性机制提供了新的见解
DOI：
10.1071/fp18163
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发表时间：
2019-01-01
影响因子：
3
通讯作者：
Liu, Yi-Ping
中科院分区：
文献类型：
--
作者：
He, Dan;Lou, Xue-Yuan;Liu, Yi-Ping
种间杂交是牡丹品种改良和育种的主要方法。Hong & J.X.但杂交不亲和性是制约种间杂交快速发展的主要因素。为了更好地了解牡丹（C.ostii cv.枫丹白）和芍药（芍药）。CV. Fenyunu），采用iTRAQ技术对授粉后24 h的柱头进行蛋白质组学分析。2900蛋白质的水平进行了定量，685蛋白质的差异表达的柱头杂交授粉后，在自花授粉。功能注释分析表明，参与RNA降解，钙信号通路，磷脂酰肌醇信号系统和有丝分裂原活化蛋白激酶（MAPK）信号通路的失调蛋白可能作出了贡献的交叉不相容性。烯醇化酶、DnaK（Heat Shock Proteins，HSP70）、GroEL（Heat Shock Proteins，HSP60）、钙调素和谷胱甘肽酶I的表达下调，腺嘌呤核苷酸转运子的表达上调，表明杂交授粉后，花粉管生长所需的能量合成、与花粉管生长极性相关的信号途径和代谢途径被阻断。选择8个基因，通过实时定量PCR确认其表达。与STRING数据库进行比较，构建了所选蛋白质的蛋白质相互作用网络。这些结果为牡丹杂交不亲和性的分子机理研究提供了基础和重要信息，并为牡丹种间杂交育种提供了理论依据。
Interspecific hybridisation is the main method for improvement and breeding of tree peony (Paeonia ostii T. Hong & J.X. Zhang), but cross-incompatibility as the major factor restricting the rapid development of interspecific hybridisation. To better understand the molecular mechanisms involved in cross-incompatibility between tree peony (Paeonia ostii cv. Fengdanbai) and herbaceous peony (Paeonia lactiflora Pall. cv. Fenyunu), a quantitative proteomic analysis using isobaric tags for relative and absolute quantitation (iTRAQ) technology was performed on the stigma 24 h after pollination. Of the 2900 proteins whose levels were quantitated, 685 proteins were differentially expressed in the stigma after hybrid pollination, in contrast to self-pollination. Functional annotation analysis showed that dysregulated proteins involved in RNA degradation, the Ca signalling pathway, the phosphatidylinositol signalling system and the mitogen-activated protein kinase (MAPK) signalling pathway may have made contributions to cross-incompatibility. The downregulated expression of enolase, DnaK (Heat Shock Proteins, HSP70), GroEL (Heat Shock Proteins, HSP60), calmodulin and glyoxalase I, and the upregulated expression of adenine nucleotide translocator indicated that the energy synthesis required by pollen tube growth, the signal pathway and the metabolic pathway related to the growth polarity of the pollen tube were blocked after hybrid pollination. Eight genes were selected to confirm their expression by quantitative real-time PCR. Compared with the STRING database, a protein-protein interaction network of the chosen proteins was constructed. These results provide fundamental and important information for research into the molecular mechanisms of cross-incompatibility in peony and should facilitate interspecific hybridisation in agricultural practice.