Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status
Expression of cell cycle-related proteins in oropharyngeal squamous cell carcinoma based on human papilloma virus status
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DOI：
10.3892/or.2017.5720
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发表时间：
2017-08-01
期刊：
影响因子：
4.2
通讯作者：
Sato, Hiroaki
中科院分区：
文献类型：
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作者：
Tsuchida, Koudai;Sugai, Tamotsu;Sato, Hiroaki
口咽鳞状细胞癌(OPSCC)中人乳头瘤病毒(HPV)和细胞周期相关蛋白(CCR)之间的分子联系尚不完全清楚。在这里，我们检测了基于HPV状态的CCR在OPSCC中的表达水平。应用原位杂交(ISH)和聚合酶链式反应(PCR)技术检测98例OPSCC中HPV感染情况，免疫组织化学方法检测CCR。在98个肿瘤中，47个肿瘤通过ISH或基于聚合酶链式反应的检测呈HPV阳性。ISH和PCR检测的一致性分析表明，在OPSCC中有很好的一致性。P16蛋白表达中等，与原位杂交检测的HPV阳性结果一致。P53、p(P)-Rb、Skp2、Cyclin和p-c-myc的高阳性率与HPV阴性的肿瘤相关，而p16和p27的高阳性率与HPV阳性的肿瘤相关。P21和细胞周期蛋白A在HPV阳性和阴性肿瘤中的阳性率无差异。最后，Ki-67在两种类型的OPSCC中普遍呈阳性表达。虽然p16的表达被认为是HPV感染的标志，但HPV的检测应采用基于ISH或PCR的方法。此外，我们关于CCRs的研究结果可能有助于理解OPSCC的致癌机制。
The molecular association between human papilloma virus (HPV) and cell cycle-related (CCR) proteins is not fully understood in oropharyngeal squamous cell carcinoma (OPSCC). Herein, we examined the expression levels of CCRs in OPSCCs based on HPV status. In situ hybridization (ISH)- and polymerase chain reaction (PCR)based assays were used to detect HPV status in 98 OPSCCs, and CCRs were detected by immunostaining. Of 98 tumors, 47 had HPV-positive tumors by either ISH- or PCR-based assays. Concordance analysis between ISH- and PCR-based tests showed a good agreement in OPSCC. Expression of p16 was moderate, consistent with HPV positivity as determined by ISH-based analysis. High p53-, phospho (p)-Rb-, SKP2-, cyclin Dl-, and p-c-myc-positive rates were correlated with HPV-negative tumors, whereas high p16- and p27-positive rates were associated with HPV-positive tumors. The positive rates for p21 and cyclin A did not differ between HPV-positive and HPV-negative tumors. Finally, Ki-67 positivity was commonly observed for both types of OPSCCs. Although expression of p16 is thought to be a marker of HPV infection, ISH- or PCR-based tests should be used for HPV detection. In addition, our results regarding CCRs may be helpful for understanding the carcinogenesis of OPSCC.