Effect of serum on dose- and temperature-dependent hepatic uptake of multilamellar vesicles (MLV).
Effect of serum on dose- and temperature-dependent hepatic uptake of multilamellar vesicles (MLV).
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血清对多层囊泡（MLV）的剂量和温度依赖性肝脏摄取的影响。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
H. Kiwada
中科院分区：
文献类型：
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作者：
K. Tahara;M. Hayashi;S. Awazu;Y. Kato;H. Kiwada
在再循环灌流的大鼠肝脏中，研究了在灌流液中加入血清对肝脏摄取多层囊泡（MLV）的影响。用膜脂质标记物（[14 C]胆固醇油酸酯）和水相标记物（[3 H]菊粉或5（6）-羧基荧光素（CF））标记MLV。[14 C]胆固醇油酸酯和CF在37 ℃下的摄取率一致，表明两种标记物均被摄取为MLV。菊糖在37 ℃下从MLV中释放，但其摄取率倾向于接近4 ℃或高MLV剂量下[14 C]胆固醇油酸酯的摄取率。血清中某些因子在37 ℃时促进MLV摄取，但在4 ℃时抑制其作用。表明促进作用可能是由于MLV吸附到肝枯否细胞表面和/或细胞吞噬作用的激活。随着MLV剂量的增加，观察到MLV摄取饱和，表明MLV吸附至肝细胞的饱和或促进MLV摄取的血清因子的消耗。
Effects of the addition of serum to the perfusate on hepatic uptake of multilamellar vesicles (MLV) were examined in recirculating perfused rat liver. MLV was labelled with both membrane lipid marker ([14C]cholesteryl oleate) and aqueous phase marker ([3H]inulin or 5(6)-carboxyfluorescein (CF)). The uptake rates of [14C]cholesteryl oleate and CF at 37 degrees C coincided well, indicating both markers to be taken up as MLV. Inulin was released from MLV at 37 degrees C but its uptake rate tended to approach that of [14C]cholesteryl oleate at 4 degrees C or at high MLV dose. Some factor in serum promoted MLV uptake at 37 degrees C, but its effect was inhibited at 4 degrees C. The promoting effect was indicated to be possibly due to activation of adsorption of MLV to the hepatic Kupffer cell surface and/or that of phagocytosis by the cells. The saturation of MLV uptake was observed with increase in MLV dose, suggesting the saturation of MLV adsorption to the hepatic cell or the consumption of serum factor which promotes MLV uptake.
DOI：
10.1016/0005-2760(81)90311-8
发表时间：
1981-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
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作者：
ABRA, RM;HUNT, CA
通讯作者：
HUNT, CA
体内脂质体分布IV：具有不同直径的连续剂量的脂质体的相互作用。
DOI：
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发表时间：
1982
期刊：
Research communications in chemical pathology and pharmacology
影响因子：
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作者：
Abra,RM;Hunt,CA
通讯作者：
Hunt,CA