Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation.
Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation.
复制标题
超灵敏传感器，具有增强的动态范围，可高速检测多色荧光辐射。
DOI：
10.1016/j.bios.2008.01.015
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发表时间：
2008
影响因子：
12.6
通讯作者：
Gorfinkel,V
中科院分区：
文献类型：
--
作者：
Tsupryk,A;Tovkach,I;Gavrilov,D;Kosobokova,O;Gudkov,G;Tyshko,G;Gorbovitski,B;Gorfinkel,V
本文介绍了用于荧光检测的新型超灵敏传感器系统的设计。系统包括两个单元：光学光谱分离单元和检测单元。传感器的光学单元对激光激发的荧光信号进行空间光谱分离，并在系统的检测部分进行光谱采集。光学部分采用衍射光栅作光谱分离元件。检测部分由32路光电倍增管和32路放大器组成，工作在单光子计数模式。该系统利用单光子探测技术和特定的信号处理算法对采集的数据进行处理，与现有的商用仪器相比，具有灵敏度高、检测速度快、线性动态范围宽等独特的特点。以新型传感器为检测装置，利用两种激光(Ar-ion和Nd-YAG)进行了DNA测序实验，获得了长达650个碱基对的DNA测序结果，其读出长度的质量因子为20。
This paper describes design of the new ultra sensitive sensor system for fluorescence detection applications. System comprises two units: optical spectra separation unit and detection unit. Optical unit of the sensor performs spatial spectra separation of signal from the laser excited fluorescence, and resulting spectra is collected in the detection part of the system. Optical part is built using diffraction grating as spectra separation element. Detection part comprises 32-channel photomultiplier tube working in single photon counting mode with our 32-channel amplifier. Using single photon detection technique and specific signal processing algorithms for collected data, the proposed system allows to achieve unique combination of characteristics—high sensitivity, high detection speed and wide linearity dynamic range comparing to existing commercial instruments. DNA sequencing experiments with new sensor as detection device, and using two types of lasers (Ar-ion and Nd-YAG) were carried out, yielding sequencing traces which have quality factor of 20 for read lengths as long as 650 base pairs.