Evaluation of mag-fura-5, the new fluorescent indicator for free magnesium measurements.
Evaluation of mag-fura-5, the new fluorescent indicator for free magnesium measurements.
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对用于游离镁测量的新型荧光指示剂 mag-fura-5 的评估。
DOI：
10.1007/bf00370418
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Lüthi,D
中科院分区：
文献类型：
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作者：
Illner,H;McGuigan,JA;Lüthi,D
评价了新的荧光指示剂mag-fura-5准确测量细胞溶质游离镁的生理变化的能力。荧光染料对Mg 2+、Mg 2 +/EGTA和Ca 2 +/EGTA溶液的表观解离常数（Kd）分别为14.7 mM、15.4 mM和1.8 mM。低Ca 2+或低H+背景的标准品与具有近似生理水平的相应样品之间的荧光比和所得pMg的计算差异不显著。而Ca ~（2+）或H ~+含量增加引起的变化有统计学意义，平均pMg差异分别为0.10±0.09（P<0.02）和0.33±0.26（P<0.01）。连续3天重复测量得到的数据具有可比性，差异不超过4%。由于这种新的荧光探针具有良好的重现性，因此，它可能适用于分离细胞中游离镁的测定。
The new fluorescent indicator, mag-fura-5, was evaluated for its ability to measure accurately physiological changes in cytosolic free magnesium. The apparent dissociation constants (Kd) of the fluorochrome for Mg2+, Mg2+/EGTA and Ca2+/EGTA solutions were 14.7 mM, 15.4 mM, and 1.8 mM respectively. The calculated difference in the fluorescence ratios and in the resulting pMg between the standards with low-Ca2+or low H+backgrounds and the corresponding samples with approximately physiological levels were not significant. In contrast, the changes due to an increased Ca2+or H+content were statistically significant, with mean pMg differences of 0.10±0.09 (P<0.02) and 0.33±0.26 (P<0.01) respectively. Repetitive measurements on 3 consecutive days yielded comparable data with differences not exceeding 4%. Because of the good reproducibility, it is suggested that the new fluoresecent probe may be suitable for free cytosolic magnesium determinations in isolated cells.