Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation.
Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation.
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DOI：
10.1186/s13567-016-0334-0
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发表时间：
2016-04-18
影响因子：
4.4
通讯作者：
Lu CP
中科院分区：
文献类型：
--
作者：
Yi L;Wang Y;Ma Z;Lin HX;Xu B;Grenier D;Fan HJ;Lu CP
马链球菌亚种。兽疫弧菌（S.equi spp.zoepidemicus）是一种机会性病原体，给我国养猪业造成重大经济损失，也对人类健康构成威胁。先前已报道过这种细菌形成生物膜。在这项研究中，我们使用免疫蛋白质组学方法来寻找生物膜生长的马链球菌表达的免疫原性蛋白。兽疫。发现了十七种免疫反应蛋白，其中九种常见的免疫反应蛋白是在浮游和生物膜生长的细菌中鉴定出的。马链球菌的免疫原性和保护功效。在小鼠中进一步研究了兽疫菌免疫反应性 GroEL 伴侣蛋白。该蛋白在体内表达，并在马链球菌属中引发高抗体滴度。小鼠兽疫弧菌感染。马链球菌的动物挑战实验。兽疫杆菌显示，75% 的用 GroEL 蛋白免疫的小鼠受到保护。使用体外生物膜抑制测定，获得的证据表明伴侣蛋白 GroEL 可能是预防和治疗持久性马链球菌的有希望的靶标。兽疫菌生物膜感染。总之，我们的结果表明，参与生物膜形成的重组 GroEL 蛋白可以有效地刺激免疫反应，从而防止马链球菌感染。兽疫菌感染。因此，它可能是被纳入马链球菌疫苗中的候选者。兽疫菌感染。
Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus. Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria. The immunogenicity and protective efficacy of the S. equi spp. zooepidemicus immunoreactive GroEL chaperone protein was further investigated in mice. The protein was expressed in vivo and elicited high antibody titers following S. equi spp. zooepidemicus infections of mice. An animal challenge experiment with S. equi spp. zooepidemicus showed that 75% of mice immunized with the GroEL protein were protected. Using in vitro biofilm inhibition assays, evidence was obtained that the chaperonin GroEL may represent a promising target for the prevention and treatment of persistent S. equi spp. zooepidemicus biofilm infections. In summary, our results suggest that the recombinant GroEL protein, which is involved in biofilm formation, may efficiently stimulate an immune response, which protects against S. equi spp. zooepidemicus infections. It may therefore be a candidate of interest to be included in vaccines against S. equi spp. zooepidemicus infections.