Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b
Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b
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DOI：
10.1016/j.jviromet.2006.07.011
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发表时间：
2006-12-01
影响因子：
3.1
通讯作者：
Buonavoglia, Canio
中科院分区：
文献类型：
--
作者：
Decaro, Nicola;Martella, Vito;Buonavoglia, Canio
基于 TaqMan 的诊断测试已开发用于鉴定腹泻犬粪便中的犬细小病毒 2 型 (CPV-2) 菌株，包括用于鉴定基于 2 型的疫苗和野外菌株（2a、2b 和 2c 型）的小沟结合剂 (MGB) 探针测定。由于 2b 型疫苗最近在欧洲获得许可，因此开发了两种新型 MGB 检测方法来区分 2b 型疫苗和 CPV 野外毒株。此类检测方法具有高度敏感性、特异性和可重复性，可同时检测同一样本中存在的 2b 型疫苗菌株和现场菌株。这些新的检测方法将有助于解决与注射 2b 型疫苗后不久在狗的粪便中检测 2b 型菌株相关的诊断问题。 (c) 2006 Elsevier B.V. 保留所有权利。
TaqMan-based diagnostic tests have been developed for the identification of canine parvovirus type 2 (CPV-2) strains in the faeces of dogs with diarrhoea, including a minor groove binder (MGB) probe assay for identification of type 2-based vaccines and field strains (types 2a, 2b and 2c). Since type 2b vaccines have been licensed recently in Europe, two novel MGB assays were developed for discrimination between type 2b vaccines and field strains of CPV. Such assays have been found to be highly sensitive, specific and reproducible, allowing for simultaneous detection of type 2b vaccinal and field strains present in the same specimens. These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine. (c) 2006 Elsevier B.V. All rights reserved.