Infection of BALB/c A mice by spiral and coccoid forms of Helicobacter pylori.
Infection of BALB/c A mice by spiral and coccoid forms of Helicobacter pylori.
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DOI：
10.1099/00222615-46-8-657
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发表时间：
1997-08
影响因子：
3
通讯作者：
Xin Wang;E. Sturegård;R. Rupar;H. Nilsson;P. Aleljung;B. Carlén;Roger Willén;Torkel Wadström
中科院分区：
文献类型：
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作者：
Xin Wang;E. Sturegård;R. Rupar;H. Nilsson;P. Aleljung;B. Carlén;Roger Willén;Torkel Wadström
幽门螺杆菌以螺旋形和球形两种不同形态存在。本研究表明，这两种形态均可感染BALB/c A小鼠。将螺旋形和球形的CCUG 17874菌株（NCTC 11637）、25菌株和553/93菌株，以10⁸菌落形成单位（cfu）的剂量，每隔2天经口接种小鼠3次。在30周的时间内，通过对胃活检组织炎症程度进行组织学评分来追踪感染情况。在每个时间点收集血清，用于酶联免疫吸附测定（ELISA）和免疫印迹分析。所有幽门螺杆菌菌株的螺旋形和球形形态在接种1周后，其炎症评分均显著高于对照组动物。组织学证据在整个30周内持续存在。幽门和十二指肠的炎症最为严重。感染553/93菌株显示出最严重的胃炎。CCUG 17874菌株的螺旋形形态在仅4周后就产生了免疫反应，而其球形形态以及25菌株和553/93菌株（螺旋形和球形形态）在16周后在ELISA和免疫印迹中抗体反应显著增加。结论是幽门螺杆菌的螺旋形和球形形态均可在BALB/c A小鼠中引起急性胃炎。
Helicobacter pylori exists in two different morphological forms, spiral and coccoid. This study demonstrated that both forms can infect BALB/c A mice. The animals were inoculated orally three times at 2-day intervals with 10(8) cfu of both spiral and coccoid forms of strain CCUG 17874 (NCTC 11637), strain 25 and strain 553/93. Infection was followed over a 30-week period by histological scoring of the grade of inflammation in gastric biopsies. At each time point sera were collected for analysis in ELISA and immunoblot analysis. Both spiral and coccoid forms of all H. pylori strains gave significantly higher inflammation scores than a control group of animals 1 week after inoculation. The histological evidence persisted throughout the entire 30 weeks. The inflammation was most severe in the pylorus and duodenum. Infection with strain 553/93 displayed the most severe gastritis. The spiral form of strain CCUG 17874 gave an immune response after only 4 weeks, whereas its coccoid form as well as strains 25 and 553/93 (spiral and coccoid forms) gave a significant increase in antibody response in ELISA and immunoblot after 16 weeks. It is concluded that both spiral and coccoid forms of H. pylori can cause acute gastritis in BALB/c A mice.