Development and evaluation of multiple tendon injury models in the mouse
Development and evaluation of multiple tendon injury models in the mouse
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DOI：
10.1016/j.jbiomech.2012.02.022
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发表时间：
2012-05-11
影响因子：
2.4
通讯作者：
Soslowsky, Louis J.
中科院分区：
文献类型：
--
作者：
Beason, David P.;Kuntz, Andrew F.;Soslowsky, Louis J.
在基础科学研究中，小鼠已被证明是一种有利的动物模型系统，重点是帮助开发和评估潜在的治疗方法;然而，小鼠肌腱的小尺寸使得一致和可重复的损伤模型和随后的生物力学评估对研究肌腱愈合具有挑战性。在这项研究中，我们探讨了多种小鼠肌腱损伤模型的可行性和可重复性。我们的假设是切口（使用刀片）和切除（使用活检打孔器）损伤将导致肌腱材料特性的一致差异。在16周龄时，17只C57 BL/6小鼠接受手术以在趾长屈肌、跟腱或髌腱中产生缺陷。每只动物每肢均接受1-2个全层、中央宽度切口或切除损伤;每肢至少有一个肌腱未受伤。损伤的分布使得每种肌腱类型的未损伤、切口损伤和切除损伤样本的数量相当。损伤后三周，对所有动物实施安乐死，并收获肌腱用于机械测试。正如假设的那样，在损伤后三周检测到所有三种不同肌腱类型的差异。虽然所有模型都产生了产生可预测结果的损伤，但髌腱模型在损伤肌腱与天然属性相比的显著差异的数量和大小以及数据的总体方差方面最一致。这一发现提供了支持其使用的基本肌腱愈合的研究，但是，未来的工作可能会使用任何这些模型，根据其适当的具体问题正在研究。(C)2012爱思唯尔有限公司保留所有权利。
The mouse has proven to be an advantageous animal model system in basic science research focused on aiding in development and evaluation of potential treatments; however, the small size of mouse tendons makes consistent and reproducible injury models and subsequent biomechanical evaluation challenging for studying tendon healing. In this study, we investigated the feasibility and reproducibility of multiple mouse tendon injury models. Our hypothesis was that incisional (using a blade) and excisional (using a biopsy punch) injuries would result in consistent differences in tendon material properties. At 16 weeks of age, 17 C57BL/6 mice underwent surgery to create defects in the flexor digitorum longus, Achilles, or patellar tendon. Each animal received 1-2 full-thickness, central-width incisional or excisional injuries per limb; at least one tendon per limb remained uninjured. The injuries were distributed such that each tendon type had comparable numbers of uninjured, incisionally injured, and excisionally injured specimens. Three weeks after injury, all animals were euthanized and tendons were harvested for mechanical testing. As hypothesized, differences were detected for all three different tendon types at three weeks post-injury. While all models created injuries that produced predictable outcomes, the patellar tendon model was the most consistent in terms of number and size of significant differences in injured tendons compared to native properties, as well as in the overall variance in the data. This finding provides support for its use in fundamental tendon healing studies; however, future work may use any of these models, based on their appropriateness for the specific question under study. (C) 2012 Elsevier Ltd. All rights reserved.