Pyridoxine 5'-phosphate oxidase is a candidate gene responsible for hypertension in Dahl-S rats.
Pyridoxine 5'-phosphate oxidase is a candidate gene responsible for hypertension in Dahl-S rats.
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DOI：
10.1016/j.bbrc.2003.11.149
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发表时间：
2004-01
影响因子：
3.1
通讯作者：
T. Okuda;T. Sumiya;N. Iwai;T. Miyata
中科院分区：
文献类型：
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作者：
T. Okuda;T. Sumiya;N. Iwai;T. Miyata
为筛选与Dahl盐敏感大鼠（Dahl-S）高血压相关的候选基因，采用寡核苷酸芯片技术分析Dahl-S和刘易斯大鼠肾脏中差异表达的基因。我们从Dahl-S和刘易斯大鼠中获得101只F2雄性大鼠，并在盐负荷9周后的14周龄时通过遥测监测进行血压（BP）和心率的精确测量。差异表达基因的基因型与F2代大鼠血压的相关性分析表明，吡哆醇5′-磷酸氧化酶（Pnpo）和儿茶酚胺-O-甲基转移酶（Comt）与血压高度相关。然而，在Comt的情况下，Dahl-S基因型与低血压相关。短/支链酰基辅酶A脱氢酶和Sah也显示出与收缩压显著相关。本研究为Pnpo是Dahl-S大鼠高血压的候选基因提供了证据。
To identify candidate genes responsible for hypertension in Dahl salt-sensitive rats (Dahl-S), an oligonucleotide microarray analysis was performed to find differentially expressed genes in kidneys of Dahl-S and Lewis rats. We obtained 101 F2 male rats from Dahl-S and Lewis rats and performed precise measurements of blood pressure (BP) and heart rate by telemetric monitoring at 14 weeks of age after 9 weeks of salt-loading. The correlation analysis between genotypes of differentially expressed genes and BP in F2 rats indicated that pyridoxine 5′-phosphate oxidase (Pnpo) and catecholamine-O-methyltransferease (Comt) showed a highly significant association with BP. However, in the case of Comt, the Dahl-S genotype correlated with low BP. Short/branched chain acyl-CoA dehydrogenase and Sah also showed a significant association with systolic blood pressure. The present study provided evidence that Pnpo is a candidate gene responsible for hypertension in Dahl-S rats.