OSMOTIC REQUIREMENT FOR SHOOT FORMATION IN TOBACCO CALLUS
OSMOTIC REQUIREMENT FOR SHOOT FORMATION IN TOBACCO CALLUS
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DOI：
10.1111/j.1399-3054.1979.tb03182.x
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发表时间：
1979-01-01
影响因子：
6.4
通讯作者：
THORPE, TA
中科院分区：
文献类型：
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作者：
BROWN, DCW;LEUNG, DWM;THORPE, TA
烟草（Nicotiana tabacum cv.威斯康星州]愈伤组织在含有3%蔗糖（wt/vol）的培养基上生长显示出最佳生长并产生最高数量的芽;在具有较低或较高蔗糖水平的培养基上生长的培养物显示出降低的生长速率和形成芽的能力。只有当培养基中补充甘露醇以提供与3%蔗糖培养基相同的水势时，在低蔗糖培养基上生长的培养物才能产生大量的芽。甘露糖不能代替生长所需的蔗糖。向培养基中添加的Bacto-Agar、三羧酸循环中间体和氨基酸水平的增加不会促进芽的形成或替代该过程所需的碳水化合物。成功地部分取代蔗糖的芽的形成与甘露醇的要求表明，部分组织碳水化合物是在一个调节作用。这一发现被进一步解释在turgor-driven增长和细胞扩张的假说。
Tobacco [Nicotiana tabacum cv. Wisconsin] callus grown on medium containing 3% sucrose (wt/vol) shows optimum growth and produces the highest number of shoots; cultures grown on medium with lower or higher sucrose levels show a reduced growth rate and capacity to form shoots. Cultures grown on a low sucrose containing medium produce a high number of shoots only if the medium is supplemented with mannitol to give the same water potential as that of the 3% sucrose medium. Mannitol cannot replace the sucrose requirement for growth. Increased levels of Bacto-Agar, tricarboxylic acid cycle intermediates and amino acids added to the medium do not promote shoot formation or replace the carbohydrate requirement for that process. The success in partially replacing the sucrose requirement for shoot formation with mannitol suggests that part of the tissue carbohydrate is acting in an osmoregulatory role. This finding is further interpreted in terms of the hypothesis of turgor-driven growth and cell expansion.