Toxic effects of combined exposure of tritiated water and genistein on the growth and development of zebrafish and its mechanism
Toxic effects of combined exposure of tritiated water and genistein on the growth and development of zebrafish and its mechanism
复制标题
氚化水与金雀异黄素联合暴露对斑马鱼生长发育的毒性作用及其机制
DOI：
10.3389/fenvs.2022.1001504
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发表时间：
2022-11
影响因子：
4.6
通讯作者：
Yu Tu
中科院分区：
文献类型：
--
作者：
Fengmei Cui;Qixuan Zhang;Jun Wan;Liang Sun;Na Chen;Huiyuan Xue;Tianzi Wang;Fajian Luo;Qiu Chen;Yu Tu
水污染和管制是我们长期关注的重要问题。环境介质通常含有多种化合物。由于人类的活动，氚广泛存在于自然界中。染料木黄酮是一种内分泌干扰物，广泛存在于水体中。它与氚水和染料木黄酮结合时会造成损害吗？在这项研究中，斑马鱼胚胎被随机分为4组：空白对照组（单纯E3培养基），氚水暴露组（介质中终浓度为3.7102Bq/mL的氚化水），金雀异黄素暴露组（终浓度为1.4mg/L染料木黄酮）和氚水与染料木黄酮联合染毒组（3.7102Bq/mL氚水+1.4mg/L染料木素）。结果表明，单独暴露3.7102Bq/ml氚水对斑马鱼胚胎发育没有影响。但染料木素联合暴露降低了斑马鱼幼鱼的存活率、孵化率和心率，并使胚胎畸形率、凋亡细胞和氧化应激水平升高。RNA测序结果表明，氚水和染料木素联合暴露影响了斑马鱼胚胎基因表达。差异表达基因主要集中在p53信号通路、类固醇激素合成、过氧化物酶体增殖物激活受体（PPAR）信号通路、细胞色素P450代谢外源物质等多个途径。qRT-PCR和基因敲除实验结果表明cyp 19 a1 b基因可能在联合暴露的毒性效应中起重要作用。
Water pollution and control are important issues of our lasting concern. Environmental media often contains a variety of compounds. Tritium is widely present in nature due to human activities. As an endocrine disruptor, genistein is widely found in water body. Will it cause damage when combined with tritiated water and genistein? In this study, Zebrafish embryos were randomly divided into 4 groups: blank control group (simple E3 medium), tritiated water exposure group (tritiated water with a final concentration of 3.7102Bq/mL in the medium), and genistein exposure group (the final concentration of 1.4mg/L genistein in the medium) and the combined exposure group of tritiated water and genistein (3.7102Bq/mL tritium water +1.4mg/L genistein). The results show that tritiated water with 3.7102Bq/ml exposure alone did not affect the development of zebrafish embryos. However, the survival rate, hatching rate and heart rate of zebrafish larvae decreased combined exposure with genistein, and the abnormality rate and apoptotic cells in the embryos and the level of oxidative stress increased. The results of RNA sequencing showed that the combined exposure of tritiated water and genistein affected the gene expression of zebrafish embryos. Differential genes were mainly enriched in many pathways, such as p53 signaling pathway, steroid hormone biosynthesis, PPAR signaling pathway, metabolism of xenobiotics by cytochrome P450. The results of qRT-PCR and gene knockout experiment showed that cyp19a1b gene may plays an important role in the toxic effects of combined exposure.
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DOI：
10.1016/j.bbagrm.2014.06.005
发表时间：
2015-02
影响因子：
4.7
作者：
Bondesson, Maria;Hao, Ruixin;Lin, Chin-Yo;Williams, Cecilia;Gustafsson, Jan-Ake
通讯作者：
Gustafsson, Jan-Ake
影响因子：
2.5
作者：
Peterson, RS;Lee, DW;Schlinger, BA
通讯作者：
Schlinger, BA
影响因子：
6
作者：
Henson HE;Taylor MR
通讯作者：
Taylor MR
影响因子：
4.1
作者：
Lukas Mustajärvi;I. Nybom;Ann;Kristin Eriksson;Wiklund;E. Eek;G. Cornelissen;Anna Sobeka
通讯作者：
Lukas Mustajärvi;I. Nybom;Ann;Kristin Eriksson;Wiklund;E. Eek;G. Cornelissen;Anna Sobeka
影响因子：
4.1
作者：
Francis K. Migwi;J. Ogunah;J. Kiratu
通讯作者：
Francis K. Migwi;J. Ogunah;J. Kiratu