A Transcriptomic Study of Maternal Thyroid Adaptation to Pregnancy in Rats.
A Transcriptomic Study of Maternal Thyroid Adaptation to Pregnancy in Rats.
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大鼠母体甲状腺对妊娠适应的转录组学研究
DOI：
10.3390/ijms161126030
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发表时间：
2015-11-13
影响因子：
5.6
通讯作者：
Fu YS
中科院分区：
文献类型：
--
作者：
Liu JL;Wang TS;Zhao M;Peng Y;Fu YS
甲状腺疾病在孕妇中相对常见。然而，妊娠对母体甲状腺的影响尚未得到系统评价。在本研究中，使用大鼠作为动物模型，我们观察到，在妊娠后期，母体甲状腺重量增加约18%。为了深入了解分子机制，我们利用RNA-seq方法来研究母体甲状腺中的整体基因表达变化。我们共鉴定了615个差异表达基因，其中大部分（558个基因或90.7%）在妊娠晚期与非妊娠对照组相比表达上调。基因本体分析显示，在上调表达的基因中，参与细胞周期和代谢的基因明显富集。出乎意料的是，途径分析显示，甲状腺激素合成途径的关键组分的表达水平没有显著改变。此外，通过检测上调基因的启动子区域，我们确定MAZ（MYC相关锌指蛋白）和TFCP 2（转录因子CP 2）为两个致病转录因子。我们的研究有助于增加对母亲甲状腺对妊娠的适应性的认识。
Thyroid disorders are relatively frequently observed in pregnant women. However, the impact of pregnancy on maternal thyroid has not been systematically evaluated. In the present study, using the rat as an animal model, we observed that the weight of maternal thyroid increased by about 18% in late pregnancy. To gain an insight into the molecular mechanisms, we took advantage of RNA-seq approaches to investigate global gene expression changes in the maternal thyroid. We identified a total of 615 differentially expressed genes, most of which (558 genes or 90.7%) were up-regulated in late pregnancy compared to the non-pregnant control. Gene ontology analysis showed that genes involved in cell cycle and metabolism were significantly enriched among up-regulated genes. Unexpectedly, pathway analysis revealed that expression levels for key components of the thyroid hormone synthesis pathway were not significantly altered. In addition, by examining of the promoter regions of up-regulated genes, we identified MAZ (MYC-associated zinc finger protein) and TFCP2 (transcription factor CP2) as two causal transcription factors. Our study contributes to an increase in the knowledge on the maternal thyroid adaptation to pregnancy.