Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased
Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased
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人类大肠（正常和患病）中碱性磷酸酶的组织化学展示
DOI：
10.1007/bf00495731
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
L. Williams
中科院分区：
文献类型：
--
作者：
L. Bell;L. Williams
用组织化学方法研究了正常和病变人大肠中碱性磷酸酶的活性。组织持续保持在4°C或以下。样品在液氮中冷冻，冷冻干燥，并包埋在2 μ的甲基丙烯酸乙二醇酯中进行切片，或在10 μ的冰甲醛钙中固定进行冷冻切片。以α-萘酰磷酸钠和萘酚AS-BI磷酸为底物，偶氮染料同时偶联染色，结果表明，正常和移行期粘膜表面上皮细胞、中、上隐窝均有碱性磷酸酶活性。
Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4° C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 μ, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 μ. The simultaneous coupling azo dye method using the substrates sodium α-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.