Evidence for oxidative stress in the hepatic mitochondria of bile duct ligated rats
Evidence for oxidative stress in the hepatic mitochondria of bile duct ligated rats
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DOI：
10.1006/phrs.1997.0225
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发表时间：
1997-09-01
影响因子：
9.3
通讯作者：
AykacToker, G
中科院分区：
文献类型：
--
作者：
Alptekin, N;Mehmetcik, G;AykacToker, G
胆管结扎 (BDL) 大鼠的肝脏匀浆和肝线粒体部分中的脂质过氧化均增加。尽管肝脏中线粒体超氧化物歧化酶 (SOD) 活性没有变化，但与假手术大鼠相比，BDL 大鼠肝线粒体部分的谷胱甘肽 (GSH) 水平和谷胱甘肽过氧化物酶 (GSH-Px) 活性降低。此外，BDL 大鼠肝脏线粒体后部分的 GSH-Px 和谷胱甘肽转移酶 (GST) 活性下降，但 SOD 活性保持不变。然而，BDL 大鼠的红细胞脂质过氧化物和 GSH 水平没有变化。总之，我们的结果表明，氧化-抗氧化平衡的紊乱，尤其是线粒体中的氧化-抗氧化平衡的紊乱，可能是 BDL 大鼠胆汁淤积损伤的原因。 (C) 1997 年意大利药理学会。
Lipid peroxidation increased both in the liver homogenates and in the hepatic mitochondrial fraction of bile duct ligated (BDL)-rats. Although mitochondrial superoxide dismutase (SOD) activity did not change in the liver, glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity decreased in hepatic mitochondrial fraction of BDL-rats as compared to sham-operated rats. In addition, GSH-Px and glutathione transferase (GST) activities decreased but SOD activity remained unchanged in the post-mitochondrial fraction of the liver from BDL-rats. However, erythrocyte lipid peroxide and GSH levels did not change in BDL-rats. In conclusion, our results show that the disturbance of oxidant-antioxidant balance especially in mitochondria may be responsible for cholestatic injury in BDL-rats. (C) 1997 The Italian Pharmacological Society.