Silver/dendrimer nanocomposites as biomarkers: Fabrication, characterization, in vitro toxicity, and intracellular detection
Silver/dendrimer nanocomposites as biomarkers: Fabrication, characterization, in vitro toxicity, and intracellular detection
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DOI：
10.1021/nl051077u
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发表时间：
2005-11-01
期刊：
影响因子：
10.8
通讯作者：
Balogh, LP
中科院分区：
文献类型：
--
作者：
Lesniak, W;Bielinska, AU;Balogh, LP
我们合成了水溶性、生物相容性、荧光且稳定的银/树枝状聚合物纳米复合材料，具有体外细胞标记的潜力。氨基-、羟基-和羧基-封端的乙二胺核心第5代聚（酰胺基胺）树枝状聚合物用于在7.4的生物pH下制备水性银（I）-树枝状聚合物络合物（具有25 Ag+/树枝状聚合物的摩尔比）。银（I）-树枝状聚合物复合物转化为树枝状聚合物纳米复合材料通过用UV光照射溶液以将结合的Ag+阳离子还原为零价Ag-0原子来实现，所述零价Ag-0原子同时被捕获在树枝状聚合物网络中，导致形成{（Ag-0）（25）-PAMAM_E5.NH2}、{（Ag-0）（25）-PAMAM_E5.NGly}、{（Ag-0）（25）-PAMAM_E5.NH2}、{（Ag-0）（25）-PAMAM_E5.NGly}、{（Ag-0）（25）-PAMAM_E5.NH2}、{（Ag-0）（25）-PAMAM_E5.NGly}、{（Ag-0）（25）-PAMAM_E5.NH2}、{（Ag-0）（25）-PAMAM和{（Ag-0）（25）-PAMAM_E5.NSAH}树枝状聚合物纳米复合物（DNC）。银-DNC的特征在于通过紫外-可见光谱，荧光光谱，动态光散射，zeta电位测量，高分辨率透射电子显微镜，X-射线能量色散谱，和选区电子衍射。使用细胞活力的XTT比色测定法评估树枝状聚合物和相关银纳米复合材料的细胞毒性。通过透射电子显微镜和共聚焦显微镜检查纳米颗粒的细胞摄取。结果表明，{（Ag-0）（25）-PAMAM_E5.NH2}、{（Ag-0）（25）-PAMAM-E5.NGly}和{（Ag-0）（25）-PAMAM_E5.NSAH}主要形成直径在3和7 nm之间的单个颗粒。树枝状聚合物纳米复合材料是荧光的，并且它们的表面电荷、细胞内化、毒性和细胞标记能力由树枝状聚合物模板的表面官能度决定。{（Ag-0）（25）-PAMAM_E5.NH2}和{（Ag-0）（25）-PAMAM_E5.NSAH}纳米复合材料显示出作为细胞生物标志物的潜在应用。
We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(l)-dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(l)-dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag-0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag-0)(25)-PAMAM_E5.NH2}, {(Ag-0)(25)-PAMAM_E5.NGly}, and {(Ag-0)(25)-PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver-DNCs were characterized by means of UV-vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag-0)(25)-PAMAM_E5.NH2}, {(Ag0-)(25)PAMAM-E5.NGly}, and {(Ag-0)(25)-PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag-0)(25)-PAMAM_E5.NH2} and {(Ag-0)(25)-PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.