An experimental study on the therapy of infantile hemangioma with recombinant interferon γ
An experimental study on the therapy of infantile hemangioma with recombinant interferon γ
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DOI：
10.1016/j.jpedsurg.2010.09.056
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发表时间：
2011-03-01
影响因子：
2.4
通讯作者：
Ji, Yi
中科院分区：
文献类型：
--
作者：
Peng, Qiang;Liu, Wenying;Ji, Yi
背景/目的：本研究的目的是研究干扰素γ（IFN-γ）治疗婴儿血管瘤的效果。方法：将4月龄女婴手术切除的血管瘤组织分成小块（4×4×5mm（3））并皮下植入裸鼠（每只小鼠2个）。 32 个存活的血管瘤块被随机分为 2 组：IFN-γ 给药组 (16) 和对照组 (16)。皮下注射γ干扰素或生理盐水，监测裸鼠血管瘤的生长情况。采用免疫组化和实时聚合酶链反应检测血管瘤的增殖和凋亡情况。结果：注射IFN-γ后7天，IFN-γ给药组的血管瘤明显小于对照组（P < .01）。 IFN-γ组的增殖细胞因子Ki-67 mRNA显着低于对照组（P < .05）。 IFN-γ组的DAPK-1 mRNA显着高于对照组(P < .05)。 IFN-γ组细胞凋亡表达显着高于对照组(P < .05)。结论：外源性IFN-γ能够有效治疗裸鼠模型血管瘤。其机制与抑制血管瘤增殖、加速血管瘤凋亡密切相关。 (C) 2011 Elsevier Inc. 保留所有权利。
Background/Purpose: The purpose of this investigation was to study the effect of interferon gamma (IFN-gamma) in the treatment of infantile hemangioma.Methods: Hemangioma tissue excised from a 4-month-old female infant who underwent surgery were separated into small nubs (4 x 4 x 5mm(3)) and implanted subcutaneously into nude mice (2 nubs per mouse). Thirty-two surviving hemangioma nubs were randomly divided into 2 groups, an IFN-gamma-administered group (16) and a control group (16). Interferon gamma or saline solution was injected subcutaneously, and the growth of hemangioma in the nude mice was monitored. Proliferation and apoptosis of hemangioma were tested by immunohistochemistry and real-time polymerase chain reaction.Results: Seven days after IFN-gamma injection, the hemangiomas in the IFN-gamma-administered group were significantly smaller than that in the control group (P < .01). The proliferation cytokine Ki-67 mRNA in the IFN-gamma group was significantly lower than that in the control group (P < .05). DAPK-1 mRNA in the IFN-gamma group was significantly higher than that in the control group (P < .05). Cell apoptosis expression in the IFN-gamma group was significantly more than that in controls (P < .05).Conclusions: Exogenous IFN-gamma can treat hemangioma effectively in a nude mice model. Its mechanism was closely related to both inhibition of hemangioma proliferation and acceleration of its apoptosis. (C) 2011 Elsevier Inc. All rights reserved.