Apricot melanoidins prevent oxidative endothelial cell death by counteracting mitochondrial oxidation and membrane depolarization.
Apricot melanoidins prevent oxidative endothelial cell death by counteracting mitochondrial oxidation and membrane depolarization.
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DOI：
10.1371/journal.pone.0048817
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Pintus G
中科院分区：
文献类型：
--
作者：
Cossu A;Posadino AM;Giordo R;Emanueli C;Sanguinetti AM;Piscopo A;Poiana M;Capobianco G;Piga A;Pintus G
富含水果和蔬菜的饮食对心血管的好处被认为是由于新鲜植物材料中含有的植物化学物质。然而，经过加工的植物食品是否提供了与未经加工的食品相同的好处，这是一个悬而未决的问题。用比色法和褐变指数法对热加工杏仁中的类黑素进行了分离，证实了类黑素的存在。内皮细胞的氧化损伤是心血管疾病(CVD)发生和发展的关键步骤，因此，探讨杏类黑素对过氧化氢诱导的人内皮细胞线粒体氧化损伤和细胞死亡的保护作用。用氧化还原敏感的荧光蛋白(RoGFP)检测细胞质和线粒体的氧化还原状态，用荧光染料JC-1检测线粒体膜电位。ECS暴露于过氧化氢后，可剂量依赖性地诱导线粒体和细胞质氧化。此外，检测到过氧化氢诱导的现象是基质金属蛋白酶的消散和内皮细胞的死亡。杏类黑素可显著拮抗并最终消除过氧化氢诱导的细胞内氧化、线粒体去极化和细胞死亡。在这方面，我们目前的结果清楚地表明，从热加工杏子中提取的类黑蛋白可以保护人体内皮细胞免受氧化应激的伤害。
The cardiovascular benefits associated with diets rich in fruit and vegetables are thought to be due to phytochemicals contained in fresh plant material. However, whether processed plant foods provide the same benefits as unprocessed ones is an open question. Melanoidins from heat-processed apricots were isolated and their presence confirmed by colorimetric analysis and browning index. Oxidative injury of endothelial cells (ECs) is the key step for the onset and progression of cardiovascular diseases (CVD), therefore the potential protective effect of apricot melanoidins on hydrogen peroxide-induced oxidative mitochondrial damage and cell death was explored in human ECs. The redox state of cytoplasmic and mitochondrial compartments was detected by using the redox-sensitive, fluorescent protein (roGFP), while the mitochondrial membrane potential (MMP) was assessed with the fluorescent dye, JC-1. ECs exposure to hydrogen peroxide, dose-dependently induced mitochondrial and cytoplasmic oxidation. Additionally detected hydrogen peroxide-induced phenomena were MMP dissipation and ECs death. Pretreatment of ECs with apricot melanoidins, significantly counteracted and ultimately abolished hydrogen peroxide-induced intracellular oxidation, mitochondrial depolarization and cell death. In this regard, our current results clearly indicate that melanoidins derived from heat-processed apricots, protect human ECs against oxidative stress.
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影响因子：
8.1
作者：
Dittrich, R.;Dragonas, C.;Pischetsrieder, M.
通讯作者：
Pischetsrieder, M.
影响因子：
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Hell, Ruediger
影响因子：
15.3
作者：
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通讯作者：
Lerici, CR
影响因子：
0.6
作者：
Ishiwata, K;Yamaguchi, T;Matoba, I
通讯作者：
Matoba, I