Action of glycosyl transferases upon "Bombay" (Oh) erythrocytes. Conversion to cells showing blood-group H and A specificities.
Action of glycosyl transferases upon "Bombay" (Oh) erythrocytes. Conversion to cells showing blood-group H and A specificities.
复制标题
糖基转移酶对“孟买”（Oh）红细胞的作用。
DOI：
10.1111/j.1432-1033.1975.tb02266.x
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
H. Tuppy
中科院分区：
文献类型：
--
作者：
H. Schenkel‐Brunner;R. Prohaska;H. Tuppy
由于遗传缺陷，罕见的“孟买”(Oh) 血型表型个体缺乏 α(1-2) 岩藻糖基转移酶，该酶负责将 H 血型前体物质转化为 H 特异性结构。用从O个体胃粘膜制备的GDP-岩藻糖和α(1-2)岩藻糖基转移酶进行处理，将天然或无花果素处理的“孟买”红细胞转化为表型类似于O细胞的细胞。然而，这种转化是在“孟买”红细胞与神经氨酸酶预先孵育后实现的，这表明这些细胞表面上的血型 H 前体分子被唾液酸残基掩盖。除了α-岩藻糖残基外，通过酶促转移α-N-乙酰半乳糖胺残基，赋予经神经氨酸酶处理的“Bombay”细胞A血型特异性。
Individuals of the rare "Bombay" (Oh) blood-group phenotype lacking, due to a genetic defect, the alpha(1-2)fucosyl transferase, which is responsible for converting blood-group H precursor substances to H-specific structures. Treatment with GDP-fucose and alpha(1-2)fucosyl transferase prepared from gastric mucosa of O individuals to transform native or ficin-treated "Bombay" erythrocytes into cells phenotypically resembling O cells. The transformation was achieved, however, after prior incubation of the "Bombay" erythrocytes with neuraminidase, indicating that blood-group H precursor molecules on the surface of these cells are masked by sialyl residues. Blood-group A specificity was conferred upon neuraminidase-treated "Bombay" cells by enzymatic transfer of alpha-N-acetylgalactosamine residues, in addition to alpha-fucose residues.